Title:
DEVICE AND METHOD FOR FREEZE DRYING BIOLOGICAL SAMPLES
Document Type and Number:
WIPO Patent Application WO/2019/142047
Kind Code:
A3
Abstract:
Methods and devices for freeze-drying a biological sample of mammalian cells or tissue are disclosed. A method may include placing a biological sample on or in a structure such as a pre-cooled metal surface to decrease a temperature of the biological sample and with the biological sample in a closed chamber applying a vacuum to the chamber to lower a pressure within the chamber, cooling the chamber to lower a temperature within the chamber and applying heat to the biological sample within the chamber. The biological sample can include one or more of stem cells, hematopoietic stem cells, mesenchymal stem cells, embryonic stem cells, induced pluripotent stem cells, sperm, oocytes, embryos, ovarian tissue, uterine tissue or testicular tissue. A method for rehydrating samples is also disclosed.
Inventors:
ARAV AMIR (IL)
Application Number:
PCT/IB2019/000037
Publication Date:
September 26, 2019
Filing Date:
January 11, 2019
Export Citation:
Assignee:
FERTILESAFE LTD (IL)
International Classes:
A01N1/02
Other References:
SHINICHI HOCHI ET AL: "Challenging Endeavour for Preservation of Freeze-Dried Mammalian Spermatozoa", JOURNAL OF REPRODUCTION AND DEVELOPMENT, vol. 57, no. 5, 1 January 2011 (2011-01-01), JP, pages 557 - 563, XP055594664, ISSN: 0916-8818, DOI: 10.1262/jrd.11-061O
ARAV AMIR ET AL: "Directional freezing of sperm and associated derived technologies", ANIMAL REPRODUCTION SCIENCE, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 169, 6 February 2016 (2016-02-06), pages 6 - 13, XP029534187, ISSN: 0378-4320, DOI: 10.1016/J.ANIREPROSCI.2016.02.007
JULIA CHRISTINA KASPER ET AL: "The freezing step in lyophilization: Physico-chemical fundamentals, freezing methods and consequences on process performance and quality attributes of biopharmaceuticals", EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, ELSEVIER SCIENCE PUBLISHERS B.V., AMSTERDAM, NL, vol. 78, no. 2, 14 March 2011 (2011-03-14), pages 248 - 263, XP028203399, ISSN: 0939-6411, [retrieved on 20110321], DOI: 10.1016/J.EJPB.2011.03.010
ARAV AMIR ET AL: "High post-thaw survival of ram sperm after partial freeze-drying", JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, PLENUM PUBLISHING, US, vol. 35, no. 7, 14 March 2018 (2018-03-14), pages 1149 - 1155, XP036556746, ISSN: 1058-0468, [retrieved on 20180314], DOI: 10.1007/S10815-018-1145-1
ARAV AMIR ET AL: "Directional freezing of sperm and associated derived technologies", ANIMAL REPRODUCTION SCIENCE, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 169, 6 February 2016 (2016-02-06), pages 6 - 13, XP029534187, ISSN: 0378-4320, DOI: 10.1016/J.ANIREPROSCI.2016.02.007
JULIA CHRISTINA KASPER ET AL: "The freezing step in lyophilization: Physico-chemical fundamentals, freezing methods and consequences on process performance and quality attributes of biopharmaceuticals", EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, ELSEVIER SCIENCE PUBLISHERS B.V., AMSTERDAM, NL, vol. 78, no. 2, 14 March 2011 (2011-03-14), pages 248 - 263, XP028203399, ISSN: 0939-6411, [retrieved on 20110321], DOI: 10.1016/J.EJPB.2011.03.010
ARAV AMIR ET AL: "High post-thaw survival of ram sperm after partial freeze-drying", JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, PLENUM PUBLISHING, US, vol. 35, no. 7, 14 March 2018 (2018-03-14), pages 1149 - 1155, XP036556746, ISSN: 1058-0468, [retrieved on 20180314], DOI: 10.1007/S10815-018-1145-1
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