Title:
DEVICE FOR PRODUCING ARTIFICIAL SKIN USING CELLS DIFFERENTIATED FROM INDUCED PLURIPOTENT STEM CELLS
Document Type and Number:
WIPO Patent Application WO/2024/010279
Kind Code:
A1
Abstract:
The present invention relates to artificial skin using fibroblasts and keratinocytes, and, more specifically, to a device for producing artificial skin using cells differentiated from induced pluripotent stem cells. In this regard, the device for producing artificial skin using cells differentiated from induced pluripotent stem cells comprises: a 3D printer (100) that can independently transport a flat plate (120) on orthogonally arranged X, Y, and Z axes; a fibroblast solution container (150), fixedly mounted on the top, for storing fibroblasts differentiated from induced pluripotent stem cells (iPSCs); a keratinocyte solution container (140), positioned adjacent to the fibroblast solution container (150), for storing keratinocytes differentiated from induced pluripotent stem cells (iPSCs); a first transport tube (145) with one end connected to the keratinocyte solution container (140) and the other end connected to a spraying device (160); a second transport tube (155) with one end connected to the fibroblast solution container (150) and the other end connected to the spraying device (160); and the spraying device (160) equipped with a first nozzle and a second nozzle wherein the first nozzle is connected with the first and second transport tubes (145, 155) independently at the top and with the first transport tube (145) at the bottom, and the second nozzle is connected with the second transport tube (155).
More Like This:
Inventors:
CHUNG HYUNG MIN (KR)
KIM C-YOON (KR)
LEE SEUL-GI (KR)
CHUNG GUJIN (KR)
KIM C-YOON (KR)
LEE SEUL-GI (KR)
CHUNG GUJIN (KR)
Application Number:
PCT/KR2023/009097
Publication Date:
January 11, 2024
Filing Date:
June 29, 2023
Export Citation:
Assignee:
CLECELL CO LTD (KR)
International Classes:
C12M3/00; A61L27/38; A61L27/60; B33Y30/00; C12M1/00; C12N5/071; C12N5/077
Foreign References:
| KR20120128774A | 2012-11-28 | |||
| KR102543340B1 | 2023-06-16 |
Other References:
ALBANNA MOHAMMED, BINDER KYLE W., MURPHY SEAN V., KIM JAEHYUN, QASEM SHADI A., ZHAO WEIXIN, TAN JOSH, EL-AMIN IDRIS B., DICE DENNI: "In Situ Bioprinting of Autologous Skin Cells Accelerates Wound Healing of Extensive Excisional Full-Thickness Wounds", SCIENTIFIC REPORTS, vol. 9, no. 1, 1 December 2019 (2019-12-01), pages 1856, XP093001147, DOI: 10.1038/s41598-018-38366-w
KIM YENA, PARK NARAE, RIM YERI ALICE, NAM YOOJUN, JUNG HYERIN, LEE KIJUN, JU JI HYEON: "Establishment of a complex skin structure via layered co-culture of keratinocytes and fibroblasts derived from induced pluripotent stem cells", STEM CELL RESEARCH & THERAPY, vol. 9, no. 1, 1 December 2018 (2018-12-01), XP055922809, DOI: 10.1186/s13287-018-0958-2
DAIKUARA LUCIANA Y., CHEN XIFANG, YUE ZHILIAN, SKROPETA DANIELLE, WOOD FIONA M., FEAR MARK W., WALLACE GORDON G.: "3D Bioprinting Constructs to Facilitate Skin Regeneration", ADVANCED FUNCTIONAL MATERIALS, WILEY - V C H VERLAG GMBH & CO. KGAA, DE, vol. 32, no. 3, 1 January 2022 (2022-01-01), DE , pages 2105080, XP093125712, ISSN: 1616-301X, DOI: 10.1002/adfm.202105080
KIM YENA, PARK NARAE, RIM YERI ALICE, NAM YOOJUN, JUNG HYERIN, LEE KIJUN, JU JI HYEON: "Establishment of a complex skin structure via layered co-culture of keratinocytes and fibroblasts derived from induced pluripotent stem cells", STEM CELL RESEARCH & THERAPY, vol. 9, no. 1, 1 December 2018 (2018-12-01), XP055922809, DOI: 10.1186/s13287-018-0958-2
DAIKUARA LUCIANA Y., CHEN XIFANG, YUE ZHILIAN, SKROPETA DANIELLE, WOOD FIONA M., FEAR MARK W., WALLACE GORDON G.: "3D Bioprinting Constructs to Facilitate Skin Regeneration", ADVANCED FUNCTIONAL MATERIALS, WILEY - V C H VERLAG GMBH & CO. KGAA, DE, vol. 32, no. 3, 1 January 2022 (2022-01-01), DE , pages 2105080, XP093125712, ISSN: 1616-301X, DOI: 10.1002/adfm.202105080
Attorney, Agent or Firm:
IPUS PATENT & LAW FIRM (KR)
Download PDF: