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Title:
DEVICE WITH SPECIFIC NUMBER OF CELL(S) AND NUCLEIC ACIDS IN THE WELLS AND TESTING/CALIBRATION METHOD USING THE DEVICE
Document Type and Number:
WIPO Patent Application WO/2019/093528
Kind Code:
A1
Abstract:
Provided is a device including at least one well and an amplifiable reagent contained in a specific copy number in the at least one well. In a preferable mode, the device includes information on the specific copy number of the amplifiable reagent. In a more preferable mode, the device includes information on uncertainty as the information on the specific copy number, and the information on uncertainty includes a coefficient of variation CV of the amplifiable reagent, and the coefficient of variation CV satisfies a relational expression: CV

Inventors:
IZUMI SATOSHI (JP)
SEO MANABU (JP)
KAWASHIMA YUDAI (JP)
OSAKI YUSUKE (JP)
Application Number:
PCT/JP2018/042041
Publication Date:
May 16, 2019
Filing Date:
November 13, 2018
Export Citation:
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Assignee:
RICOH CO LTD (JP)
IZUMI SATOSHI (JP)
SEO MANABU (JP)
KAWASHIMA YUDAI (JP)
OSAKI YUSUKE (JP)
International Classes:
C12Q1/6851; C12M1/00; C12Q1/686; G01N35/00; G01N35/02; G06M11/00
Domestic Patent References:
WO2015151511A12015-10-08
WO2008021419A22008-02-21
Foreign References:
US9361426B22016-06-07
JP2018099109A2018-06-28
EP3415608A22018-12-19
JP2014033658A2014-02-24
JP2015195735A2015-11-09
Other References:
QIANG ZHANG ET AL: "Development of a facile droplet-based single-cell isolation platform for cultivation and genomic analysis in microorganisms", SCIENTIFIC REPORTS, vol. 7, no. 1, 23 January 2017 (2017-01-23), XP055518191, DOI: 10.1038/srep41192
SANGJUN ET AL., PLOS ONE, vol. 6, no. 3, pages e17455
Attorney, Agent or Firm:
HIROTA, Koichi (JP)
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