Title:
DIFFERENTIATION CONTROL METHOD FOR PLURIPOTENT STEM CELLS
Document Type and Number:
WIPO Patent Application WO/2018/143312
Kind Code:
A1
Abstract:
This differentiation control method for pluripotent stem cells is characterized by: selecting laminin or a fragment thereof using binding affinity to the pluripotent stem cells as an index, the binding affinity being determinable by temporally observing the survival rate and mobility of the cells; and inducing the differentiation of the pluripotent stem cells in the presence of the laminin or a fragment thereof. The present invention allows easy production, from pluripotent stem cells, of a cell population in which the ratio of differentiation-induced cells is arbitrarily changed. A cell population obtained by the production method is highly useful for treatment of diseases by cellular therapy.
Inventors:
NISHIDA KOHJI (JP)
SEKIGUCHI KIYOTOSHI (JP)
HAYASHI RYUHEI (JP)
SHIBATA SHUN (JP)
SEKIGUCHI KIYOTOSHI (JP)
HAYASHI RYUHEI (JP)
SHIBATA SHUN (JP)
Application Number:
PCT/JP2018/003315
Publication Date:
August 09, 2018
Filing Date:
January 31, 2018
Export Citation:
Assignee:
UNIV OSAKA (JP)
International Classes:
C12N5/0735; C07K14/47; C12N5/071; C12N5/079; C12N5/0793
Domestic Patent References:
| WO2015068505A1 | 2015-05-14 | |||
| WO2016114285A1 | 2016-07-21 |
Foreign References:
| JP2014513924A | 2014-06-19 |
Other References:
SHIBATA, SHUN ET AL.: "Laminin isoform controls generation of eye cell from iPS cell", REGENERATIVE MEDICINE, vol. 16, 1 February 2017 (2017-02-01), pages 270, XP009516426, ISSN: 1347-7919
TAKAYAMA, KAZUO ET AL.: "Induction of differentiation from human iPS cell utilizing laminin isoform to hepatocyte or bile duct epithelial cell", ANNUAL PROGRAM AND ABSTRACTS OF 39TH CONFERENCE OF THE MOLECULAR BIOLOGY SOCIETY OF JAPAN, 16 November 2016 (2016-11-16), pages 2P-0315, XP009516461
SEKIGUCHI, KIYOTOSHI: "Development of cultivation substrate for stem cell on the basis of diversity of extracellular matrix", REGENERATIVE MEDICINE, vol. 14, 1 February 2015 (2015-02-01), pages 142, XP009516427, ISSN: 1347-7919
ANONYMOUS: "Functional analysis of basement membrane protein laminin in stem cell differentiation", SCIENTIFIC RESEARCH FUNDS RAISING BUSINESS DATABASE, 21 April 2016 (2016-04-21), pages 1 - 1, XP009518605, Retrieved from the Internet [retrieved on 20180417]
NEDO: "Creation technique development of model cell for research", NEDO 2008 MIDDLE ANNUAL REPORT: ANALYSIS TECHNIQUE DEVELOPMENT OF GENE FUNCTION USING MODEL CELL , 15 May 2009 (2009-05-15), pages 1 - 8, XP009516887, Retrieved from the Internet [retrieved on 20180417]
YAMAGUCHI, M. ET AL.: "Adhesion, Migration, and Proliferation of Cultured Human Corneal Endothelial Cells by Laminin-5", INVEST. OPHTHALMOL. VIS. SCI., vol. 52, no. 2, 3 February 2011 (2011-02-03), pages 679 - 684, XP055177805
HAYASHI ET AL., NATURE, vol. 531, no. 7594, 17 March 2016 (2016-03-17), pages 376 - 80
EDGAR D ET AL., J. CELL BIOL., vol. 105, 1987, pages 589 - 598
HIROYUKI IDO ET AL., THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 282, 2007, pages 11144 - 11154
HIROYUKI IDO ET AL., THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 279, 2004, pages 10946 - 10954
MATRIX BIOL., vol. 25, no. 3, April 2006 (2006-04-01), pages 189 - 97
GU J ET AL., THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 277, 2002, pages 19922 - 19928
J CELL BIOL., vol. 205, no. 2, 28 April 2014 (2014-04-28), pages 251 - 63
TAKAYAMA, KAZUO ET AL.: "Induction of differentiation from human iPS cell utilizing laminin isoform to hepatocyte or bile duct epithelial cell", ANNUAL PROGRAM AND ABSTRACTS OF 39TH CONFERENCE OF THE MOLECULAR BIOLOGY SOCIETY OF JAPAN, 16 November 2016 (2016-11-16), pages 2P-0315, XP009516461
SEKIGUCHI, KIYOTOSHI: "Development of cultivation substrate for stem cell on the basis of diversity of extracellular matrix", REGENERATIVE MEDICINE, vol. 14, 1 February 2015 (2015-02-01), pages 142, XP009516427, ISSN: 1347-7919
ANONYMOUS: "Functional analysis of basement membrane protein laminin in stem cell differentiation", SCIENTIFIC RESEARCH FUNDS RAISING BUSINESS DATABASE, 21 April 2016 (2016-04-21), pages 1 - 1, XP009518605, Retrieved from the Internet
NEDO: "Creation technique development of model cell for research", NEDO 2008 MIDDLE ANNUAL REPORT: ANALYSIS TECHNIQUE DEVELOPMENT OF GENE FUNCTION USING MODEL CELL , 15 May 2009 (2009-05-15), pages 1 - 8, XP009516887, Retrieved from the Internet
YAMAGUCHI, M. ET AL.: "Adhesion, Migration, and Proliferation of Cultured Human Corneal Endothelial Cells by Laminin-5", INVEST. OPHTHALMOL. VIS. SCI., vol. 52, no. 2, 3 February 2011 (2011-02-03), pages 679 - 684, XP055177805
HAYASHI ET AL., NATURE, vol. 531, no. 7594, 17 March 2016 (2016-03-17), pages 376 - 80
EDGAR D ET AL., J. CELL BIOL., vol. 105, 1987, pages 589 - 598
HIROYUKI IDO ET AL., THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 282, 2007, pages 11144 - 11154
HIROYUKI IDO ET AL., THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 279, 2004, pages 10946 - 10954
MATRIX BIOL., vol. 25, no. 3, April 2006 (2006-04-01), pages 189 - 97
GU J ET AL., THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 277, 2002, pages 19922 - 19928
J CELL BIOL., vol. 205, no. 2, 28 April 2014 (2014-04-28), pages 251 - 63
Attorney, Agent or Firm:
IWATANI, Ryo (JP)
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