Title:
DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS IN AVIAN EMBRYOS
Document Type and Number:
WIPO Patent Application WO2003048344
Kind Code:
A3
Abstract:
The invention relates to a method of preparing from human embryonic stem cells, differentiated cells suitable for transplantation, by introducing human embryonic stems cells into an avian host embryo. Also provided is a method of directing the differentiation of human embryonic stem cells by introducing them into a selected location in an avian host embryo, which dictates their differentiation pattern. The invention provides normal, transplantable differentiated human cells, e.g. progenitor and other cells, particularly neural cells. The invention also relates to therapeutic and diagnostic methods employing the differentiated cells of the invention.
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Inventors:
GOLDSTEIN RON (IL)
BENVENISTY NISSIM (IL)
DRUKKER MICHA (IL)
BENVENISTY NISSIM (IL)
DRUKKER MICHA (IL)
Application Number:
PCT/IL2002/000978
Publication Date:
October 16, 2003
Filing Date:
December 05, 2002
Export Citation:
Assignee:
YISSUM RES DEV CO (IL)
UNIV BAR ILAN (IL)
GOLDSTEIN RON (IL)
BENVENISTY NISSIM (IL)
DRUKKER MICHA (IL)
UNIV BAR ILAN (IL)
GOLDSTEIN RON (IL)
BENVENISTY NISSIM (IL)
DRUKKER MICHA (IL)
International Classes:
C12N5/0735; C12N15/873; A61K35/12; (IPC1-7): C12N5/06; A61K35/12
Other References:
FONTAINE-PERUS JOSIANE ET AL: "Mouse-chick chimera: A developmental model of murine neurogenic cells.", DEVELOPMENT (CAMBRIDGE), vol. 124, no. 16, 1997, pages 3025 - 3036, XP002234871, ISSN: 0950-1991
GAJOVIC S ET AL: "DIFFERENTIATION OF THE MOUSE EMBRYOID BODIES GRAFTED ON THE CHORIOALLANTOIC MEMBRANE OF THE CHICK EMBRYO", INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY, UNIVERSITY OF THE BASQUE COUNTRY PRES, LEIOA, ES, vol. 42, no. 2, March 1998 (1998-03-01), pages 225 - 228, XP001056263, ISSN: 0214-6282
TUAN R S ET AL: "Developmental Biology Protocols", METHODS IN MOLECULAR BIOLOGY, HUMANA PRESS INC., CLIFTON, NJ, US, vol. 1, 2000, pages 439 - 446, XP002198678
GOLDSTEIN RONALD S ET AL: "Integration and differentiation of human embryonic stem cells transplanted to the chick embryo.", DEVELOPMENTAL DYNAMICS, vol. 225, no. 1, September 2002 (2002-09-01), September, 2002, pages 80 - 86, XP009007834, ISSN: 1058-8388
RATHJEN P D ET AL: "PROPERTIES AND USES OF EMBRYONIC STEM CELLS: PROSPECTS FOR APPLICATION TO HUMAN BIOLOGY AND GENE THERAPY", REPRODUCTION, FERTILITY AND DEVELOPMENT, CSIRO, EAST MELBOURNE, AU, vol. 10, no. 1, 1998, pages 31 - 47, XP000916997, ISSN: 1031-3613
EIGES RACHEL ET AL: "Establishment of human embryonic stem cell-transfected clones carrying a marker for undifferentiated cells.", CURRENT BIOLOGY, vol. 11, no. 7, 3 April 2001 (2001-04-03), pages 514 - 518, XP002228082, ISSN: 0960-9822
GAJOVIC S ET AL: "DIFFERENTIATION OF THE MOUSE EMBRYOID BODIES GRAFTED ON THE CHORIOALLANTOIC MEMBRANE OF THE CHICK EMBRYO", INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY, UNIVERSITY OF THE BASQUE COUNTRY PRES, LEIOA, ES, vol. 42, no. 2, March 1998 (1998-03-01), pages 225 - 228, XP001056263, ISSN: 0214-6282
TUAN R S ET AL: "Developmental Biology Protocols", METHODS IN MOLECULAR BIOLOGY, HUMANA PRESS INC., CLIFTON, NJ, US, vol. 1, 2000, pages 439 - 446, XP002198678
GOLDSTEIN RONALD S ET AL: "Integration and differentiation of human embryonic stem cells transplanted to the chick embryo.", DEVELOPMENTAL DYNAMICS, vol. 225, no. 1, September 2002 (2002-09-01), September, 2002, pages 80 - 86, XP009007834, ISSN: 1058-8388
RATHJEN P D ET AL: "PROPERTIES AND USES OF EMBRYONIC STEM CELLS: PROSPECTS FOR APPLICATION TO HUMAN BIOLOGY AND GENE THERAPY", REPRODUCTION, FERTILITY AND DEVELOPMENT, CSIRO, EAST MELBOURNE, AU, vol. 10, no. 1, 1998, pages 31 - 47, XP000916997, ISSN: 1031-3613
EIGES RACHEL ET AL: "Establishment of human embryonic stem cell-transfected clones carrying a marker for undifferentiated cells.", CURRENT BIOLOGY, vol. 11, no. 7, 3 April 2001 (2001-04-03), pages 514 - 518, XP002228082, ISSN: 0960-9822
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