Title:
DIGITAL HOLOGRAPHIC MICROSCOPE
Document Type and Number:
WIPO Patent Application WO/2018/070451
Kind Code:
A1
Abstract:
Provided is a digital holographic microscope in which two digital holographic microscopes for detecting a fluorescence image and a phase image, respectively, are combined to be able to three-dimensionally measure a fluorescence image and a phase image at the same time, and perform measurement at a high SN ratio in all the polarization states including random light polarization. The digital holographic microscope is provided with: a first holographic optical system that, by using laser light 5, acquires a phase three-dimensional image due to interference light generated by superimposing object light which passes through a sample stage 4 and reference light which does not pass through the sample stage 4 onto each other; and a second holographic optical system that, by using fluorescent excitation light 6, acquires a fluorescence three-dimensional image due to a fluorescence signal light, wherein phase measurement by the first holographic optical system and fluorescence measurement by the second holographic optical system are performed at the same time. The second holographic optical system includes a bifocal lens 2 on which a diffraction grating 7 is superposed. Each of the bifocal lens and the diffraction grating has polarization dependency. The SN ratio is enhanced at a time of self-interference of the fluorescence signal light so that a fluorescence three-dimensional image is acquired.
Inventors:
MATOBA OSAMU (JP)
Application Number:
PCT/JP2017/036894
Publication Date:
April 19, 2018
Filing Date:
October 11, 2017
Export Citation:
Assignee:
UNIV KOBE NAT UNIV CORP (JP)
International Classes:
G02B21/00; G01N21/64; G02B5/18; G03H1/00
Domestic Patent References:
| WO2016163560A1 | 2016-10-13 |
Foreign References:
| JP2004538451A | 2004-12-24 | |||
| JP2015001726A | 2015-01-05 | |||
| JP2000098243A | 2000-04-07 |
Other References:
QUAN, XIANGYU ET AL.: "Yasuhiro, Prototyping of Hybrid Digital Holographic Microscope Capable of Fluorescence and Phase Imaging", OPTICS & PHOTONICS JAPAN 2014, 5 November 2014 (2014-11-05), pages 15
Attorney, Agent or Firm:
THE PATENT CORPORATE BODY GLOBAL INTELLECTUAL PROPERTY (JP)
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