Title:
DIGITAL SOMATIC CELL VARIATION ANALYSIS
Document Type and Number:
WIPO Patent Application WO/2020/218554
Kind Code:
A1
Abstract:
The present disclosure provides a method for analyzing the composition of variations in a tissue, simply, at low cost, and/or with high precision. The method for analyzing the composition of cells in a tissue according the present disclosure comprises a step for assessing information about the sequence of cells from samples containing amplified nucleic acid derived from single cells in the tissue. This method enables assessment of the cell composition in a tissue simply at low cost when being carried out before whole genome sequencing, and enables improvement in precision since it is possible to conduct comparison of long gene sequences when information regarding the target to be analyzed is narrowed down from digital sequence information after whole genome sequencing is performed.
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Inventors:
HOSOKAWA MASAHITO (JP)
TAKEYAMA HARUKO (JP)
NISHIKAWA YOHEI (JP)
TAKEYAMA HARUKO (JP)
NISHIKAWA YOHEI (JP)
Application Number:
PCT/JP2020/017793
Publication Date:
October 29, 2020
Filing Date:
April 24, 2020
Export Citation:
Assignee:
BITBIOME INC (JP)
International Classes:
C12Q1/6809; C12M1/00; C12Q1/6806; C12Q1/6886; G01N35/08; G01N37/00
Domestic Patent References:
| WO2019216271A1 | 2019-11-14 |
Foreign References:
| US20160068899A1 | 2016-03-10 | |||
| US20180371525A1 | 2018-12-27 |
Other References:
HOSOKAWA, M. ET AL.: "Massively parallel whole genome amplification for single- cell sequencing using droplet microfluidics", SCIENTIFIC REPORTS, vol. 7, 2017, pages 5199, XP055672497, DOI: 10.1038/s41598-017-05436-4
MASATO KOGAWA, HOSOKAWA MASAHITO, NISHIKAWA YOHEI, MORI KAZUKI, TAKEYAMA HARUKO: "Obtaining high-quality draft genomes from uncultured microbes by cleaning and co-assembly of single-cell amplified genomes", SCIENTIFIC REPORTS, vol. 8, no. 1, 1 December 2018 (2018-12-01), XP055759180, DOI: 10.1038/s41598-018-20384-3
RIEKA CHIJIIWA, HOSOKAWA MASAHITO, KOGAWA MASATO, NISHIKAWA YOHEI, IDE KEIGO, SAKANASHI CHIKAKO, TAKAHASHI KAI, TAKEYAMA HARUKO: "Single-cell genomics of uncultured bacteria reveals dietary fiber responders in the mouse gut microbiota", MICROBIOME, vol. 8, no. 1, 1 December 2020 (2020-12-01), XP055759172, DOI: 10.1186/s40168-019-0779-2
GENG, T. ET AL.: "Single- Cell Forensic Short Tandem Repeat Typing within Microfluidic Droplets", ANALYTICAL CHEMISTRY, vol. 86, 22 November 2013 (2013-11-22), pages 703 - 712, XP055711588, DOI: 10.1021/ac403137h
ZHANG, H. ET AL.: "Massively Parallel Single- Molecule and Single- Cell Emulsion Reverse Transcription Polymerase Chain Reaction Using Agarose Droplet Microfluidics", ANALYTICAL CHEMISTRY, vol. 84, 27 March 2012 (2012-03-27), pages 3599 - 3606, XP002745401, DOI: 10.1021/ac2033084
NOVAK, R. ET AL.: "Single- Cell Multiplex Gene Detection and Sequencing with Microfluidically Generated Agarose Emulsions", ANGEWANDTE CHEMIE INTERNATIONAL EDITION, vol. 50, 2011, pages 390 - 395, XP002629259, DOI: 10.1002/ANIE.201006089
MASATO KOGAWA, HOSOKAWA MASAHITO, NISHIKAWA YOHEI, MORI KAZUKI, TAKEYAMA HARUKO: "Obtaining high-quality draft genomes from uncultured microbes by cleaning and co-assembly of single-cell amplified genomes", SCIENTIFIC REPORTS, vol. 8, no. 1, 1 December 2018 (2018-12-01), XP055759180, DOI: 10.1038/s41598-018-20384-3
RIEKA CHIJIIWA, HOSOKAWA MASAHITO, KOGAWA MASATO, NISHIKAWA YOHEI, IDE KEIGO, SAKANASHI CHIKAKO, TAKAHASHI KAI, TAKEYAMA HARUKO: "Single-cell genomics of uncultured bacteria reveals dietary fiber responders in the mouse gut microbiota", MICROBIOME, vol. 8, no. 1, 1 December 2020 (2020-12-01), XP055759172, DOI: 10.1186/s40168-019-0779-2
GENG, T. ET AL.: "Single- Cell Forensic Short Tandem Repeat Typing within Microfluidic Droplets", ANALYTICAL CHEMISTRY, vol. 86, 22 November 2013 (2013-11-22), pages 703 - 712, XP055711588, DOI: 10.1021/ac403137h
ZHANG, H. ET AL.: "Massively Parallel Single- Molecule and Single- Cell Emulsion Reverse Transcription Polymerase Chain Reaction Using Agarose Droplet Microfluidics", ANALYTICAL CHEMISTRY, vol. 84, 27 March 2012 (2012-03-27), pages 3599 - 3606, XP002745401, DOI: 10.1021/ac2033084
NOVAK, R. ET AL.: "Single- Cell Multiplex Gene Detection and Sequencing with Microfluidically Generated Agarose Emulsions", ANGEWANDTE CHEMIE INTERNATIONAL EDITION, vol. 50, 2011, pages 390 - 395, XP002629259, DOI: 10.1002/ANIE.201006089
Attorney, Agent or Firm:
YAMAMOTO Shusaku et al. (JP)
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