Title:
DILUENT FOR FLUORESCENT NANO PARTICLES, KIT FOR IMMUNOFLUORESCENT STAINING WHICH UTILIZES SAME, SOLUTION FOR IMMUNOFLUORESCENT STAINING, IMMUNOFLUORESCENT STAINING METHOD, AND GENE STAINING METHOD
Document Type and Number:
WIPO Patent Application WO/2016/093268
Kind Code:
A1
Abstract:
[Problem] To provide a means for, in immunostaining using fluorescent nano particles, preventing the occurrence of non-specific adsorption of the fluorescent nano particles to reduce background noises, thereby detecting and quantifying a biological substance of interest with higher accuracy.
[Solution] Immunostaining is carried out by diluting fluorescent nano particles with a diluent for fluorescent nano particles, wherein the diluent for fluorescent nano particles contains 1 to 5% (W/W) of a protein having a molecular weight of 40,000 or more (e.g., BSA) and 1 to 3% (W/W) of a protein having a molecular weight of less than 40,000 (e.g., casein). In the diluent, when casein is used as the low-molecular-weight protein, it is preferred that the content of κ-casein in the casein is 10% (W/W) or less and the ratio of the content of α-casein to the content of β-casein, i.e., an α-casein:β-casein ratio, is (40 to 60):(60 to 40) (wherein the total amount of α-casein and β-casein is 100).
Inventors:
GOUDA HIDEKI (JP)
TAKANASHI KENSAKU (JP)
TAKANASHI KENSAKU (JP)
Application Number:
PCT/JP2015/084500
Publication Date:
June 16, 2016
Filing Date:
December 09, 2015
Export Citation:
Assignee:
KONICA MINOLTA INC (JP)
International Classes:
G01N33/53; G01N21/64; G01N33/48; G01N33/536
Domestic Patent References:
| WO2012029342A1 | 2012-03-08 | |||
| WO2014136885A1 | 2014-09-12 |
Foreign References:
| JP2012194013A | 2012-10-11 | |||
| JPH08320323A | 1996-12-03 | |||
| JP2014142348A | 2014-08-07 | |||
| JPH09196922A | 1997-07-31 | |||
| JP2006053031A | 2006-02-23 | |||
| JP2014066720A | 2014-04-17 | |||
| JP2013088296A | 2013-05-13 | |||
| JP2002202312A | 2002-07-19 |
Other References:
See also references of EP 3232195A4
Attorney, Agent or Firm:
SSINPAT PATENT FIRM (JP)
Patent business corporation SSINPAT (JP)
Patent business corporation SSINPAT (JP)
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