Title:
DIPHOSPHOMEVALONATE DECARBOXYLASE VARIANT AND METHOD FOR MANUFACTURING OLEFIN COMPOUND USING SAME
Document Type and Number:
WIPO Patent Application WO/2018/143163
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a method which can be used to highly productively manufacture an olefin compound, and an enzyme for use in the method. According to the present invention, mutations involving amino acid substitution to various sites of diphosphomevalonate decarboxylase (MVD) were introduced to prepare multiple MVD variants. The variants were evaluated in terms of catalytic activity involved in production of an olefin compound such as isoprene, and as a result, it was found that the catalytic activity improves when serine at position 153 and threonine at position 209 are each substituted with another amino acid. It was also revealed that the catalytic activities of the variants further improve by additionally substituting glycine at position 152 with another amino acid.
Inventors:
ORISHIMO RYOKO (JP)
SHIRAI TOMOKAZU (JP)
TAKAHASHI KAZUHIRO (JP)
HIZA MISAO (JP)
TANABE YUSUKE (JP)
SHIRAI TOMOKAZU (JP)
TAKAHASHI KAZUHIRO (JP)
HIZA MISAO (JP)
TANABE YUSUKE (JP)
Application Number:
PCT/JP2018/002875
Publication Date:
August 09, 2018
Filing Date:
January 30, 2018
Export Citation:
Assignee:
RIKEN (JP)
ZEON CORP (JP)
YOKOHAMA RUBBER CO LTD (JP)
ZEON CORP (JP)
YOKOHAMA RUBBER CO LTD (JP)
International Classes:
C12N15/09; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/88; C12P5/02
Domestic Patent References:
| WO2015021045A2 | 2015-02-12 | |||
| WO2017022804A1 | 2017-02-09 | |||
| WO2015004211A2 | 2015-01-15 | |||
| WO2013092567A2 | 2013-06-27 | |||
| WO2015004211A2 | 2015-01-15 | |||
| WO2015021045A2 | 2015-02-12 |
Foreign References:
| JP2015501660A | 2015-01-19 |
Other References:
GOGERTY, D. S. ET AL.: "Formation of Isobutene from 3-Hydroxy-3-Methylbutyrate by Diphosphomevalonate Decarboxylase", APPL. ENVIRON. MICROBIOL., vol. 76, no. 24, 2010, pages 8004 - 8010, XP002680645, ISSN: 0099-2240
ORISHITA, RYOKO ET AL.: "Substrate-specific modifications of useful enzymes, and application to production of bio-isoprene", LECTURE ABSTRACTS IN 2016 MEETING OF JAPAN SOCIETY OF BIOSCIENCE , BIOTECHNOLOGY, AND AGROCHEMISTRY, vol. 2016, 5 March 2016 (2016-03-05), pages 2J003, XP009508887, ISSN: 2186-7976
ORISHIMO RYOKO ET AL.: "Modification of Substrate Specificity of Useful Enzymes and Application to Bioisoprene Production", LECTURE ABSTRACT COLLECTION FOR THE ANNUAL MEETING OF THE JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY, vol. 2016, 5 March 2016 (2016-03-05), pages 2J003
TETRAHEDRON LETTERS, vol. 20, no. 15, 1988, pages 1763 - 1766
KUNKEL, T. A., PROC NATL ACAD SCI USA, vol. 82, no. 2, 1985, pages 488 - 492
HO, S. N.HUNT, H. D.HORTON, R. M.PULLEN, J. K.PEASE, L. R., GENE, vol. 77, 1989, pages 51 - 59
See also references of EP 3578649A4
ORISHITA, RYOKO ET AL.: "Substrate-specific modifications of useful enzymes, and application to production of bio-isoprene", LECTURE ABSTRACTS IN 2016 MEETING OF JAPAN SOCIETY OF BIOSCIENCE , BIOTECHNOLOGY, AND AGROCHEMISTRY, vol. 2016, 5 March 2016 (2016-03-05), pages 2J003, XP009508887, ISSN: 2186-7976
ORISHIMO RYOKO ET AL.: "Modification of Substrate Specificity of Useful Enzymes and Application to Bioisoprene Production", LECTURE ABSTRACT COLLECTION FOR THE ANNUAL MEETING OF THE JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY, vol. 2016, 5 March 2016 (2016-03-05), pages 2J003
TETRAHEDRON LETTERS, vol. 20, no. 15, 1988, pages 1763 - 1766
KUNKEL, T. A., PROC NATL ACAD SCI USA, vol. 82, no. 2, 1985, pages 488 - 492
HO, S. N.HUNT, H. D.HORTON, R. M.PULLEN, J. K.PEASE, L. R., GENE, vol. 77, 1989, pages 51 - 59
See also references of EP 3578649A4
Attorney, Agent or Firm:
CENTCREST IP ATTORNEYS (JP)
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