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Title:
DISEASE DETECTION METHOD
Document Type and Number:
WIPO Patent Application WO/2019/013341
Kind Code:
A1
Abstract:
The method according to the present invention comprises measuring the amount of a peptide as a biomarker contained in a biological sample with the use of LC-MS and detecting a specific disease on the basis of the measurement result, wherein pretreatment steps to be conducted before the measurement with the use of LC-MS involve: a step for adding to the biological sample a stable isotope reagent, said reagent being obtained by labeling a peptide with a stable isotope, and trifluoroacetic acid to give a mixed liquid sample; a step for boiling the mixed liquid sample; a step for pouring the boiled mixed liquid sample into a solid phase extraction column and allowing the solid phase extraction column to hold the peptide; and a step for passing a water-soluble organic solvent through the solid phase extraction column and thus eluting the peptide held by the solid phase extraction column.

Inventors:
KAWAKAMI DAISUKE (JP)
MATSUBARA TOSHIYA (JP)
UCHIDA KAZUHIKO (JP)
MENO KOHJI (JP)
Application Number:
PCT/JP2018/026582
Publication Date:
January 17, 2019
Filing Date:
July 13, 2018
Export Citation:
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Assignee:
SHIMADZU CORP (JP)
MCBI INC (JP)
International Classes:
G01N30/06; G01N27/62; G01N30/08; G01N30/72; G01N30/88; G01N33/68; C07K14/47
Domestic Patent References:
WO2016044697A12016-03-24
Foreign References:
JP2016028244A2016-02-25
JP2005511063A2005-04-28
JP2008514946A2008-05-08
JP2010071900A2010-04-02
JP2006308533A2006-11-09
JP2006284389A2006-10-19
Other References:
BIOTAGE JAPAN LTD.: "Extraction of Peptides From Human Plasma and Serum Using EVOLUTE® ABN 96-Well Plates Prior to LC-MS/MS ", 2012, pages 1 - 5, XP009518981, Retrieved from the Internet
Attorney, Agent or Firm:
KYOTO INTERNATIONAL PATENT LAW OFFICE (JP)
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