Title:
DISEASE MODEL ANIMAL EXPRESSING MEGSIN/RAGE/iNOS AND METHOD OF EVALUATING COMPOUND WITH THE USE OF THE ANIMAL
Document Type and Number:
WIPO Patent Application WO/2005/055706
Kind Code:
A1
Abstract:
A triple Tg (megsin/RAGE/iNOS-Tg) is constructed by crossing a megsin-Tg with an RAGE/iNOS-Tg. It is found out that this megsin/RAGE/iNOS-Tg suffers from the onset of remarkable diabetic kidney lesion at an early stage which has never been observed so far and shows various uniform lesion images such as glomerular enlargement. It is also found out that the animal showing these symptoms is useful as a model animal of diabetic nephropathy. That is to say, the disease model animal as described above overexpresses megsin gene, receptor for advanced glycation end product gene and inducible nitric oxide synthase gene and, therefore, suffers from renal dysfunction associated with glomerular disorders at an early stage.
Inventors:
MIYATA TOSHIO (JP)
KUROKAWA KIYOSHI (JP)
YAMAMOTO HIROSHI (JP)
OKAMOTO HIROSHI (JP)
KUROKAWA KIYOSHI (JP)
YAMAMOTO HIROSHI (JP)
OKAMOTO HIROSHI (JP)
Application Number:
PCT/JP2004/018413
Publication Date:
June 23, 2005
Filing Date:
December 09, 2004
Export Citation:
Assignee:
JAPAN SCIENCE & TECH AGENCY (JP)
GENE THESIS CO LTD (JP)
MIYATA TOSHIO (JP)
KUROKAWA KIYOSHI (JP)
YAMAMOTO HIROSHI (JP)
OKAMOTO HIROSHI (JP)
GENE THESIS CO LTD (JP)
MIYATA TOSHIO (JP)
KUROKAWA KIYOSHI (JP)
YAMAMOTO HIROSHI (JP)
OKAMOTO HIROSHI (JP)
International Classes:
A01K67/027; C07K14/47; C12N9/02; C12N15/09; C12N15/85; G01N33/50; (IPC1-7): A01K67/027; C12N15/09; G01N33/50
Domestic Patent References:
| WO2001024628A1 | 2001-04-12 |
Other References:
YAMAMOTO Y. ET AL: "AGE-RAGE o hyoteki to shita Tonyobyo Kekkan Shogai Yokusei no Kanosei.", FOLIA PHARMACOLOGICA JAPONICA, vol. 121, no. 1, January 2003 (2003-01-01), pages 49 - 55, XP002990270
YAMAMOTO Y. ET AL: "Development and prevention of advanced diabetic nephropathy in RAGE-over expressing mice.", J.CLIN.INVEST., vol. 108, no. 2, July 2001 (2001-07-01), pages 261 - 268, XP002983725
TAKAMURA T. ET AL: "Transgenic mice overexpressing type 2 nitricoxide synthase in pancreatic beta cells develop insulindependent diabetes without insulitis.", J.BIOL.CHEM., vol. 273, no. 5, 30 January 1998 (1998-01-30), pages 2493 - 2496, XP002983726
See also references of EP 1712125A4
YAMAMOTO Y. ET AL: "Development and prevention of advanced diabetic nephropathy in RAGE-over expressing mice.", J.CLIN.INVEST., vol. 108, no. 2, July 2001 (2001-07-01), pages 261 - 268, XP002983725
TAKAMURA T. ET AL: "Transgenic mice overexpressing type 2 nitricoxide synthase in pancreatic beta cells develop insulindependent diabetes without insulitis.", J.BIOL.CHEM., vol. 273, no. 5, 30 January 1998 (1998-01-30), pages 2493 - 2496, XP002983726
See also references of EP 1712125A4
Attorney, Agent or Firm:
Shimizu, Hatsushi (1-1-1 Oroshi-machi, Tsuchiura-sh, Ibaraki 47, JP)
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