Title:
DISPLACEMENT ASSAY FOR DETECTION OF SMALL MOLECULES
Document Type and Number:
WIPO Patent Application WO2003089902
Kind Code:
A3
Abstract:
Complex of an anti-cocaine aptamer and the dye diethylthiotricarbocyanine behaves as a colorimetric sensor with attenuation in absorbance at 760 nm for cocaine in the concentration range of 2-5000 µM. Mechanistic studies indicate an intermolecular displacement of the dye as the mechanism of action of the sensor. As the dye is insoluble in buffer, cocaine binding can be detected as displaced dye precipitates and supernatant decolorizes.
Inventors:
STOJANOVIC MILAN N
LANDRY DONALD W
LANDRY DONALD W
Application Number:
PCT/US2003/012095
Publication Date:
June 10, 2004
Filing Date:
April 18, 2003
Export Citation:
Assignee:
UNIV COLUMBIA (US)
International Classes:
A01N61/00; C07H21/02; C07H21/04; C07K1/00; C07K16/00; C12M1/34; C12Q1/00; C12Q1/68; C12Q1/70; G01N33/53; G01N33/566; G01N; (IPC1-7): C12Q1/00; C12Q1/70; C12Q1/68; G01N33/53; C07H21/02; C07H21/04; C07K1/00; C07K16/00; A01N61/00
Foreign References:
| US5597696A | 1997-01-28 | |||
| US6472153B1 | 2002-10-29 | |||
| US5270163A | 1993-12-14 |
Other References:
ULRICH ET AL.: "In vitro selection of RNA molecules that displace cocaine from the membrane-bound nicotinic acetylcholine receptor", PROC. NATL. ACAD. SCI. USA, vol. 95, November 1998 (1998-11-01), pages 14051 - 14056, XP002972608
STOJANOVIC ET AL.: "Aptamer based folding fluorescent sensor for cocaine", J. AM. CHEM. SOC., vol. 123, 4 May 2001 (2001-05-04), pages 4928 - 4931, XP002225605
STOJANOVIC ET AL.: "Fluorescent sensors based on aptamer self-assembly", J. AM. CHEM. SOC., vol. 122, 2 November 2000 (2000-11-02), pages 11547 - 11548, XP002225604
STOJANOVIC ET AL.: "Aptamer based folding fluorescent sensor for cocaine", J. AM. CHEM. SOC., vol. 123, 4 May 2001 (2001-05-04), pages 4928 - 4931, XP002225605
STOJANOVIC ET AL.: "Fluorescent sensors based on aptamer self-assembly", J. AM. CHEM. SOC., vol. 122, 2 November 2000 (2000-11-02), pages 11547 - 11548, XP002225604
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