Title:
DNA APTAMER CAPABLE OF BINDING TO NON-SMALL CELL LUNG CANCER CELL (H1975)
Document Type and Number:
WIPO Patent Application WO/2016/129531
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing a novel DNA aptamer useful for the diagnosis and treatment of lung cancer, the prevention of metastasis of lung cancer and the like.
A DNA aptamer which is characterized by having at least one nucleotide sequence selected from sequences respectively represented by SEQ ID NOs: 1 and 2, and is also characterized by being capable of binding specifically to a non-small cell lung cancer cell; a composition for detecting a lung cancer cell, which contains the DNA aptamer; a kit for detecting a lung cancer cell, which contains the DNA aptamer; a method for detecting lung cancer, which is characterized by using the DNA aptamer; a detection method comprising a step of bringing the DNA aptamer into contact with a sample collected from a living body, selected from the group consisting of a lung cell, a lung tissue, blood, serum, plasma, saliva and sputum and a step of observing a response associated with the binding of the sample to the DNA aptamer to detect the presence of a lung cancer cell; a pharmaceutical composition for preventing the metastasis of lung cancer or treating lung cancer, which contains the DNA aptamer; and a drug delivery system for preventing the metastasis of lung cancer or treating lung cancer, which contains the DNA aptamer.
Inventors:
FURUSHO HITOSHI (JP)
Application Number:
PCT/JP2016/053560
Publication Date:
August 18, 2016
Filing Date:
February 05, 2016
Export Citation:
Assignee:
NISSAN CHEMICAL IND LTD (JP)
International Classes:
C12N15/09; A61K31/711; A61K31/713; A61P35/00; A61P35/04; C12N15/115; C12Q1/68
Foreign References:
| JP2014217311A | 2014-11-20 | |||
| JP2015019606A | 2015-02-02 | |||
| CN101914542A | 2010-12-15 | |||
| CN101914543A | 2010-12-15 | |||
| CN101955939A | 2011-01-26 |
Other References:
ZHAO, ZILONG ET AL.: "Recognition of subtype non-small cell lung cancer by DNA aptamers selected from living cells", ANALYST, vol. 134, no. 9, 2009, pages 1808 - 1814, XP009503956, ISSN: 0003-2654
XU, LI ET AL.: "Cellular internalization and cytotoxicity of aptamers selected from lung cancer cell", AM. J. BIOMED. SCI., vol. 5, no. 1, 2013, pages 47 - 58, XP055457072, ISSN: 1937-9080
KAUR J ET AL.: "Ets1 identified as a novel molecular target of RNA aptamer selected against metastatic cells for targeted delivery of nano-formulation", ONCOGENE, vol. 34, no. 41, 2015, pages 5216 - 5228, XP055457073, ISSN: 0950-9232
See also references of EP 3257940A4
XU, LI ET AL.: "Cellular internalization and cytotoxicity of aptamers selected from lung cancer cell", AM. J. BIOMED. SCI., vol. 5, no. 1, 2013, pages 47 - 58, XP055457072, ISSN: 1937-9080
KAUR J ET AL.: "Ets1 identified as a novel molecular target of RNA aptamer selected against metastatic cells for targeted delivery of nano-formulation", ONCOGENE, vol. 34, no. 41, 2015, pages 5216 - 5228, XP055457073, ISSN: 0950-9232
See also references of EP 3257940A4
Attorney, Agent or Firm:
TSUKUNI & ASSOCIATES et al. (JP)
Patent business corporation Tsukuni (JP)
Patent business corporation Tsukuni (JP)
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