Title:
DNA APTAMER SPECIFICALLY BINDING TO COVID-19 VIRUS AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2022/005048
Kind Code:
A1
Abstract:
The present invention relates to a DNA aptamer that specifically binds to a COVID-19 virus and use thereof, in which the aptamer selected by a modified SELEX (Systematic evolution of ligands by exponential enrichment) method of the present invention binds to a site different from a portion where an antibody binds to a COVID-19 virus nuclear protein, and thus can be applied to various fields such as a sandwich-type biosensor. Furthermore, such an aptamer has very good stability and sensitivity, compared to formulations comprising conventional antibodies, and is advantageous in that mass production is enabled in a short time and at low cost by using a chemical synthesis method and in that various modifications are easily made to increase a binding force.
Inventors:
KIM MIN GON (KR)
KANG JU YOUNG (KR)
PARK CHIN JU (KR)
YEOM GYU HO (KR)
KANG JU YOUNG (KR)
PARK CHIN JU (KR)
YEOM GYU HO (KR)
Application Number:
PCT/KR2021/007417
Publication Date:
January 06, 2022
Filing Date:
June 14, 2021
Export Citation:
Assignee:
SB BIOSCIENCE CO LTD (KR)
International Classes:
C12N15/115; C07K14/005; C12Q1/70
Foreign References:
| KR101291737B1 | 2013-07-31 | |||
| US9939443B2 | 2018-04-10 |
Other References:
YANLING SONG, JIA SONG, XINYU WEI, MENGJIAO HUANG, MIAO SUN, LIN ZHU, BINGQIAN LIN, HAICONG SHEN, ZHI ZHU, CHAOYONG YANG: "Discovery of Aptamers Targeting the Receptor-Binding Domain of the SARS-CoV-2 Spike Glycoprotein", ANALYTICAL CHEMISTRY, vol. 92, no. 14, 21 July 2020 (2020-07-21), US , pages 9895 - 9900, XP055759814, ISSN: 0003-2700, DOI: 10.1021/acs.analchem.0c01394
CHEN ZHIQIANG; WU QIHAN; CHEN JING; NI XIAOHUA; DAI JIANFENG: "A DNA Aptamer Based Method for Detection of SARS-CoV-2 Nucleocapsid Protein", VIROLOGICA SINICA, vol. 35, no. 3, 25 May 2020 (2020-05-25), DE , pages 351 - 354, XP037184841, ISSN: 1674-0769, DOI: 10.1007/s12250-020-00236-z
DATABASE PROTEIN 27 May 2020 (2020-05-27), ANONYMOUS: "nucleocapsid phosphoprotein [Severe acute respiratory syndrome coronavirus 2]", XP055884105, retrieved from GENBANK Database accession no. QIQ49839
CHEN ZHIQIANG; WU QIHAN; CHEN JING; NI XIAOHUA; DAI JIANFENG: "A DNA Aptamer Based Method for Detection of SARS-CoV-2 Nucleocapsid Protein", VIROLOGICA SINICA, vol. 35, no. 3, 25 May 2020 (2020-05-25), DE , pages 351 - 354, XP037184841, ISSN: 1674-0769, DOI: 10.1007/s12250-020-00236-z
DATABASE PROTEIN 27 May 2020 (2020-05-27), ANONYMOUS: "nucleocapsid phosphoprotein [Severe acute respiratory syndrome coronavirus 2]", XP055884105, retrieved from GENBANK Database accession no. QIQ49839
Attorney, Agent or Firm:
G1 IP LAW FIRM (KR)
Download PDF:
Previous Patent: MOVABLE ASPHALT HEATING DEVICE
Next Patent: ANTENNA MODULE AND ELECTRONIC DEVICE COMPRISING SAME
Next Patent: ANTENNA MODULE AND ELECTRONIC DEVICE COMPRISING SAME