Title:
DNA-BINDING DOMAIN OF CRISPR SYSTEM, NON-FUCOSYLATED AND PARTIALLY FUCOSYLATED PROTEINS, AND METHODS THEREOF
Document Type and Number:
WIPO Patent Application WO/2016/075662
Kind Code:
A3
Abstract:
The present disclosure relates to a method of obtaining a cell where fucosylation pathways are modified, leading to production of partially fucosylated and non-fucosylated protein products, specifically antibodies from the cell. The present disclosure employs the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) technology. The method of the present disclosure targets the Fut8 gene and GMD gene in a cell. Such products are used in developing therapeutics and biomarkers, and in diagnosis and prognosis of diseases.
Inventors:
PRASAD BHARGAV (IN)
UNNIKRISHNAN DIVYA (IN)
HAZARIKA JAHNABI (IN)
RODRIGUES KAVITHA IYER (IN)
GHOSH MALOY (IN)
M PAVITHRA (IN)
D PRAVIN KUMAR (IN)
BHATTACHARJEE SANGHAMITRA (IN)
M SATHYABALAN (IN)
SRINIVASAN SANKARANARAYANAN (IN)
CHATTERJEE SOHANG (IN)
MAITY SUNIT (IN)
K VEERESHA (IN)
HALAN VIVEK (IN)
B M YOGENDRA MANJUNATH (IN)
HORA ANURADHA (IN)
N BAIRAVABALAKUMAR (IN)
NAIR KARTHIKA (IN)
THANIGAIVEL ASWINI (IN)
MALIWALAVE AMOL (IN)
SHENOY BHARATH R (IN)
PENDSE RAJESHWARI (IN)
PATHAK PRABHAT KUMAR (IN)
KURUP ANISHA (IN)
RAO SAHANA BHIMA (IN)
UNNIKRISHNAN DIVYA (IN)
HAZARIKA JAHNABI (IN)
RODRIGUES KAVITHA IYER (IN)
GHOSH MALOY (IN)
M PAVITHRA (IN)
D PRAVIN KUMAR (IN)
BHATTACHARJEE SANGHAMITRA (IN)
M SATHYABALAN (IN)
SRINIVASAN SANKARANARAYANAN (IN)
CHATTERJEE SOHANG (IN)
MAITY SUNIT (IN)
K VEERESHA (IN)
HALAN VIVEK (IN)
B M YOGENDRA MANJUNATH (IN)
HORA ANURADHA (IN)
N BAIRAVABALAKUMAR (IN)
NAIR KARTHIKA (IN)
THANIGAIVEL ASWINI (IN)
MALIWALAVE AMOL (IN)
SHENOY BHARATH R (IN)
PENDSE RAJESHWARI (IN)
PATHAK PRABHAT KUMAR (IN)
KURUP ANISHA (IN)
RAO SAHANA BHIMA (IN)
Application Number:
PCT/IB2015/058777
Publication Date:
July 07, 2016
Filing Date:
November 13, 2015
Export Citation:
Assignee:
ZUMUTOR BIOLOG INC (US)
International Classes:
C12N15/113; C12N15/09; C12N15/63
Domestic Patent References:
| WO2013013013A2 | 2013-01-24 | |||
| WO2003035835A2 | 2003-05-01 | |||
| WO2015010114A1 | 2015-01-22 | |||
| WO2015052231A2 | 2015-04-16 |
Foreign References:
| EP1676860A1 | 2006-07-05 | |||
| EP1792987A1 | 2007-06-06 |
Other References:
CARLOTTA RONDA ET AL: "Accelerating genome editing in CHO cells using CRISPR Cas9 and CRISPy, a web-based target finding tool", BIOTECHNOLOGY AND BIOENGINEERING., vol. 111, no. 8, 22 August 2014 (2014-08-22), US, pages 1604 - 1616, XP055244605, ISSN: 0006-3592, DOI: 10.1002/bit.25233
LAETITIA MALPHETTES ET AL: "Highly efficient deletion of FUT8 in CHO cell lines using zinc-finger nucleases yields cells that produce completely nonfucosylated antibodies", BIOTECHNOLOGY AND BIOENGINEERING, vol. 106, no. 5, 8 April 2010 (2010-04-08), pages 774 - 783, XP055170568, ISSN: 0006-3592, DOI: 10.1002/bit.22751
KANDA ET AL: "Establishment of a GDP-mannose 4,6-dehydratase (GMD) knockout host cell line: A new strategy for generating completely non-fucosylated recombinant therapeutics", JOURNAL OF BIOTECHNOLOGY, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 130, no. 3, 19 June 2007 (2007-06-19), pages 300 - 310, XP022119693, ISSN: 0168-1656, DOI: 10.1016/J.JBIOTEC.2007.04.025
LAETITIA MALPHETTES ET AL: "Highly efficient deletion of FUT8 in CHO cell lines using zinc-finger nucleases yields cells that produce completely nonfucosylated antibodies", BIOTECHNOLOGY AND BIOENGINEERING, vol. 106, no. 5, 8 April 2010 (2010-04-08), pages 774 - 783, XP055170568, ISSN: 0006-3592, DOI: 10.1002/bit.22751
KANDA ET AL: "Establishment of a GDP-mannose 4,6-dehydratase (GMD) knockout host cell line: A new strategy for generating completely non-fucosylated recombinant therapeutics", JOURNAL OF BIOTECHNOLOGY, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 130, no. 3, 19 June 2007 (2007-06-19), pages 300 - 310, XP022119693, ISSN: 0168-1656, DOI: 10.1016/J.JBIOTEC.2007.04.025
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