Title:
DNA CODING FOR POLYPEPTIDE PARTICIPATING IN BIOSYNTHESIS OF PLADIENOLIDE
Document Type and Number:
WIPO Patent Application WO/2006/009276
Kind Code:
A1
Abstract:
A polypeptide participating in the biosynthesis of pladienolide as a macrolide compound; a DNA coding for such a polypeptide; a variant thereof; a transformant in which part or the whole of such a DNA or variant thereof has been incorporated; and a process for producing pladienolide as a macrolide compound with the use of such a transformant. In particular, there is provided an isolated pure DNA comprising at least one region coding for a polypeptide participating in the biosynthesis of pladienolide. Further, there are provided a polypeptide coded for by this DNA and a recombinant plasmid capable of self-replication or integration replication carrying this DNA. Still further, there are provided a transformant carrying this DNA and a process for producing pladienolide, characterized in that this transformant is cultured in a culture medium and pladienolide is collected from the culture solution.
Inventors:
MACHIDA KAZUHIRO
ARISAWA AKIRA
TAKEDA SUSUMU
YOSHIDA MASASHI
TSUCHIDA TOSHIO
ARISAWA AKIRA
TAKEDA SUSUMU
YOSHIDA MASASHI
TSUCHIDA TOSHIO
Application Number:
PCT/JP2005/013541
Publication Date:
January 26, 2006
Filing Date:
July 19, 2005
Export Citation:
Assignee:
EISAI CO LTD (JP)
MERCIAN CORP
MACHIDA KAZUHIRO
ARISAWA AKIRA
TAKEDA SUSUMU
YOSHIDA MASASHI
TSUCHIDA TOSHIO
MERCIAN CORP
MACHIDA KAZUHIRO
ARISAWA AKIRA
TAKEDA SUSUMU
YOSHIDA MASASHI
TSUCHIDA TOSHIO
International Classes:
C12N15/52; C07K14/36; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/00; C12N9/02; C12P17/16; (IPC1-7): C12N15/52; C07K14/36; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/00; C12N9/02; C12P17/16
Domestic Patent References:
| WO2002060890A1 | 2002-08-08 | |||
| WO2004011661A1 | 2004-02-05 | |||
| WO1993013663A1 | 1993-07-22 |
Other References:
XUE Y. ET AL.: "A gene cluster for marcolide antobiotic biosynthesis in Streptomyces venezuelae: architecture of metabolic diversity.", PROC.NATL. ACAD.SCI USA, vol. 95, no. 21, 1998, pages 12111 - 121116, XP002117399
DONADIO S. ET AL.: "An erythromycin analog produced by reprogramming of polyketide synthesis.", PROC.NATL.ACAD.SCI. USA, vol. 90, no. 15, 1993, pages 7119 - 7123, XP002044728
HOPWOOD D. ET AL.: "Molecular genetics of polyketides and its comparison to fatty acid biosynthesis.", ANNU.REV.GENET., vol. 24, 1990, pages 37 - 66, XP000929983
KATZ L. ET AL.: "Polyketide synthesis: prospects for hybrid antibiotics.", ANNU.REV.MICROBIOL., vol. 47, 1993, pages 875 - 912, XP000654850
SCIENCE, vol. 291, 2001, pages 1790 - 1792
DONADIO S. ET AL.: "An erythromycin analog produced by reprogramming of polyketide synthesis.", PROC.NATL.ACAD.SCI. USA, vol. 90, no. 15, 1993, pages 7119 - 7123, XP002044728
HOPWOOD D. ET AL.: "Molecular genetics of polyketides and its comparison to fatty acid biosynthesis.", ANNU.REV.GENET., vol. 24, 1990, pages 37 - 66, XP000929983
KATZ L. ET AL.: "Polyketide synthesis: prospects for hybrid antibiotics.", ANNU.REV.MICROBIOL., vol. 47, 1993, pages 875 - 912, XP000654850
SCIENCE, vol. 291, 2001, pages 1790 - 1792
Attorney, Agent or Firm:
Furuya, Satoshi (2-17-8 Nihonashi-Hamach, Chuo-ku Tokyo, JP)
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