Title:
DNA DETECTION APPARATUS, DNA DETECTION DEVICE AND DNA DETECTION METHOD
Document Type and Number:
WIPO Patent Application WO/2010/026950
Kind Code:
A1
Abstract:
Provided is a chemical luminescence-detecting apparatus that comprises a plate wherein a plurality of reaction cells are arranged one-dimensionally or two-dimensionally. By simultaneously filling the reaction cells with an enzyme embedded in a gel and with DNA-fixed beads, large amounts of enzyme are trapped in the reaction cells, enzyme activity is improved, and highly sensitive pyrosequencing is realized.
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Inventors:
SHIRAI, Masataka (Hitachi Ltd., 280, Higashikoigakubo 1-chome, Kokubunji-sh, Tokyo 01, 〒1858601, JP)
白井 正敬 (〒01 東京都国分寺市東恋ヶ窪一丁目２８０番地 株式会社日立製作所 中央研究所内 Tokyo, 〒1858601, JP)
KAJIYAMA, Tomoharu (Hitachi Ltd., 280, Higashikoigakubo 1-chome, Kokubunji-sh, Tokyo 01, 〒1858601, JP)
白井 正敬 (〒01 東京都国分寺市東恋ヶ窪一丁目２８０番地 株式会社日立製作所 中央研究所内 Tokyo, 〒1858601, JP)
KAJIYAMA, Tomoharu (Hitachi Ltd., 280, Higashikoigakubo 1-chome, Kokubunji-sh, Tokyo 01, 〒1858601, JP)
Application Number:
JP2009/065205
Publication Date:
March 11, 2010
Filing Date:
August 31, 2009
Export Citation:
Assignee:
HITACHI, LTD. (6-6 Marunouchi 1-chome, Chiyoda-ku Tokyo, 80, 〒1008280, JP)
株式会社日立製作所 (〒80 東京都千代田区丸の内一丁目６番６号 Tokyo, 〒1008280, JP)
SHIRAI, Masataka (Hitachi Ltd., 280, Higashikoigakubo 1-chome, Kokubunji-sh, Tokyo 01, 〒1858601, JP)
白井 正敬 (〒01 東京都国分寺市東恋ヶ窪一丁目２８０番地 株式会社日立製作所 中央研究所内 Tokyo, 〒1858601, JP)
株式会社日立製作所 (〒80 東京都千代田区丸の内一丁目６番６号 Tokyo, 〒1008280, JP)
SHIRAI, Masataka (Hitachi Ltd., 280, Higashikoigakubo 1-chome, Kokubunji-sh, Tokyo 01, 〒1858601, JP)
白井 正敬 (〒01 東京都国分寺市東恋ヶ窪一丁目２８０番地 株式会社日立製作所 中央研究所内 Tokyo, 〒1858601, JP)
International Classes:
C12M1/00; C12Q1/68; G01N21/76; G01N33/53; G01N33/543; C12N15/09
Attorney, Agent or Firm:
TSUTSUI, Yamato (Tsutsui & Associates, 3F Shinjuku Gyoen Bldg. 3-10,Shinjuku 2-chome, Shinjuku-ku, Tokyo 22, 〒1600022, JP)
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