Title:
DNA FREE CRISPR PLANT TRANSFORMATION
Document Type and Number:
WIPO Patent Application WO/2019/150200
Kind Code:
A3
Abstract:
Disclosed herein are compositions of transfection enhancing agents, including PLUS reagents, lipofectamine and/or PEG solutions for ribonucleoprotein-mediated delivery of programmable endonucleases to plants and methods of use thereof, such as plant genome engineering.
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Inventors:
PARK AIDEN (KR)
PARK SLKI (KR)
YOON JI (KR)
CHOI SUNMEE (KR)
CHOE SUNGHWA (KR)
PARK JONGJIN (KR)
PARK SLKI (KR)
YOON JI (KR)
CHOI SUNMEE (KR)
CHOE SUNGHWA (KR)
PARK JONGJIN (KR)
Application Number:
PCT/IB2019/000132
Publication Date:
September 26, 2019
Filing Date:
January 29, 2019
Export Citation:
Assignee:
G FLAS LIFE SCIENCES (KR)
PARK AIDEN Y (KR)
PARK SLKI (KR)
YOON JI YOUNG (KR)
CHOI SUNMEE (KR)
CHOE SUNGHWA (KR)
PARK AIDEN Y (KR)
PARK SLKI (KR)
YOON JI YOUNG (KR)
CHOI SUNMEE (KR)
CHOE SUNGHWA (KR)
International Classes:
C12N9/22; C12N15/82; C12N15/10; C12N15/11; C12N15/113
Domestic Patent References:
| WO2014204726A1 | 2014-12-24 |
Other References:
WOO, J. W. ET AL.: "DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins", NATURE BIOTECHNOLOGY, vol. 33, no. 11, 2015, pages 1162 - 1164, XP055290196
KIM, K. ET AL.: "Genome surgery using Cas9 ribonucleoproteins for the treatment of age-related macular degeneration", GENOME RESEARCH, vol. 27, no. 3, 2017, pages 419 - 426, XP055636224
KIM, H. ET AL.: "CRISPR/Cpfl-mediated DNA-free plant genome editing", NATURE COMMUNICATIONS, vol. 8, no. 14406, 16 February 2017 (2017-02-16), pages 1 - 7, XP002786577
YU , X. ET AL.: "Improved delivery of Cas9 protein/gRNA complexes using lipofectamine CRISPRMAX", BIOTECHNOLOGY LETTERS, vol. 38, 2016, pages 919 - 929, XP002765623
BURGER, A. ET AL.: "Maximizing mutagenesis with solubilized CRISPR-Cas9 ribonucleoprotein complexes", DEVELOPMENT, vol. 143, no. 11, 2016, pages 2025 - 2037, XP055636227
KIM, K. ET AL.: "Genome surgery using Cas9 ribonucleoproteins for the treatment of age-related macular degeneration", GENOME RESEARCH, vol. 27, no. 3, 2017, pages 419 - 426, XP055636224
KIM, H. ET AL.: "CRISPR/Cpfl-mediated DNA-free plant genome editing", NATURE COMMUNICATIONS, vol. 8, no. 14406, 16 February 2017 (2017-02-16), pages 1 - 7, XP002786577
YU , X. ET AL.: "Improved delivery of Cas9 protein/gRNA complexes using lipofectamine CRISPRMAX", BIOTECHNOLOGY LETTERS, vol. 38, 2016, pages 919 - 929, XP002765623
BURGER, A. ET AL.: "Maximizing mutagenesis with solubilized CRISPR-Cas9 ribonucleoprotein complexes", DEVELOPMENT, vol. 143, no. 11, 2016, pages 2025 - 2037, XP055636227
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