Title:
DNA METHYLATION EDITING KIT AND DNA METHYLATION EDITING METHOD
Document Type and Number:
WIPO Patent Application WO/2017/090724
Kind Code:
A1
Abstract:
A DNA methylation editing kit including: (1) a fused protein of inactivated CRISPR-associated endonuclease Cas9 (dCas9) having no nuclease activity and a tag peptide array in which a plurality of tag peptides are linked by linkers, or RNA or DNA encoding the fused protein; (2) a fused protein of a tag peptide binding site and a methylase or demethylase, or RNA or DNA encoding the fused protein; and (3) guide RNA (gRNA) that includes a sequence complementary to a DNA sequence within 1 kb of a desired site of methylation or demethylation, or DNA expressing the gRNA.
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Inventors:
HATADA IZUHO (JP)
MORITA SUMIYO (JP)
HORII TAKURO (JP)
MORITA SUMIYO (JP)
HORII TAKURO (JP)
Application Number:
PCT/JP2016/084958
Publication Date:
June 01, 2017
Filing Date:
November 25, 2016
Export Citation:
Assignee:
NAT UNIV CORP GUNMA UNIV (JP)
International Classes:
C12N15/09; C07K16/18; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/10
Domestic Patent References:
| WO2014152432A2 | 2014-09-25 |
Other References:
TANENBAUM E. M. ET AL.: "A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging", CELL, vol. 159, 2014, pages 635 - 646, XP029084861
MAEDER M. L. ET AL.: "Targeted DNA demethylation and activation of endogenous genes using programmable TALE-TET1 fusion proteins", NATURE BIOTECHNOLOGY, vol. 31, no. 12, 2013, pages 1137 - 1142, XP055402828
MALI P. ET AL.: "Cas9 as a versatile tool for engineering biology", NATURE METHODS, vol. 10, no. 10, 2013, pages 957 - 963, XP002718606
TAIGA YAMAZAKI ET AL.: "Genome Henshu Gijutsu o Oyo shita Pericentromere eno Jin'iteki -Hairetsu Tokuteki DNA Methyl-ka Yudo", BMB2015 KOEN YOSHISHU, vol. 2015, 6 November 2015 (2015-11-06), XP9510609
SHEN L. ET AL.: "A single amino acid substitution confers enhanced methylation activity of mammalian Dnmt3b on chromatin DNA", NUCLEIC ACIDS RESEARCH, vol. 38, no. 18, 2010, pages 6054 - 6064, XP055496863
CABANTOUS S. ET AL.: "A new protein-protein interaction sensor based on tripartite split- GFP association", SCIENTIFIC REPORTS, vol. 3, pages 2854/1 - 2854/9, XP055260864
SKELTON N. J. ET AL.: "Origins of PDZ domain ligand specificity", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 278, no. 9, 2003, pages 7645 - 7654, XP002454235
MORITA S. ET AL.: "Targeted DNA demethylation in vivo using dCas9-peptide repeat and scFv-TET1 catalytic domain fusions", NATURE BIOTECHNOLOGY, vol. 34, no. 10, October 2016 (2016-10-01), pages 1060 - 1065, XP055459761
MAEDER ML ET AL., NAT BIOTECHNOL, vol. 31, 2013, pages 1137 - 1142
"JIKKEN IGAKU", July 2014, YODOSHA CO., LTD., pages: 1690 - 1714
TANENBAUM ME ET AL., CELL, vol. 159, 2014, pages 635 - 646
PROTEIN ENGINEERING, DESIGN & SELECTION, vol. 24, no. 5, 2011, pages 419 - 428
CURRENT OPINION IN CHEMICAL BIOLOGY, vol. 15, 2011, pages 789 - 797
PROC. NATL. ACAD. SCI. USA, vol. 105, no. 18, 2008, pages 6578 - 6583
SHEN L; GAO G; ZHANG Y; ZHANG H; YE Z; HUANG S; HUANG J; KANG J: "A single amino acid substitution confers enhanced methylation activity of mammalian Dnmt3b on chromatin DNA", NUCLEIC ACIDS RES., vol. 38, 2010, pages 6054 - 6064
See also references of EP 3382018A4
MAEDER M. L. ET AL.: "Targeted DNA demethylation and activation of endogenous genes using programmable TALE-TET1 fusion proteins", NATURE BIOTECHNOLOGY, vol. 31, no. 12, 2013, pages 1137 - 1142, XP055402828
MALI P. ET AL.: "Cas9 as a versatile tool for engineering biology", NATURE METHODS, vol. 10, no. 10, 2013, pages 957 - 963, XP002718606
TAIGA YAMAZAKI ET AL.: "Genome Henshu Gijutsu o Oyo shita Pericentromere eno Jin'iteki -Hairetsu Tokuteki DNA Methyl-ka Yudo", BMB2015 KOEN YOSHISHU, vol. 2015, 6 November 2015 (2015-11-06), XP9510609
SHEN L. ET AL.: "A single amino acid substitution confers enhanced methylation activity of mammalian Dnmt3b on chromatin DNA", NUCLEIC ACIDS RESEARCH, vol. 38, no. 18, 2010, pages 6054 - 6064, XP055496863
CABANTOUS S. ET AL.: "A new protein-protein interaction sensor based on tripartite split- GFP association", SCIENTIFIC REPORTS, vol. 3, pages 2854/1 - 2854/9, XP055260864
SKELTON N. J. ET AL.: "Origins of PDZ domain ligand specificity", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 278, no. 9, 2003, pages 7645 - 7654, XP002454235
MORITA S. ET AL.: "Targeted DNA demethylation in vivo using dCas9-peptide repeat and scFv-TET1 catalytic domain fusions", NATURE BIOTECHNOLOGY, vol. 34, no. 10, October 2016 (2016-10-01), pages 1060 - 1065, XP055459761
MAEDER ML ET AL., NAT BIOTECHNOL, vol. 31, 2013, pages 1137 - 1142
"JIKKEN IGAKU", July 2014, YODOSHA CO., LTD., pages: 1690 - 1714
TANENBAUM ME ET AL., CELL, vol. 159, 2014, pages 635 - 646
PROTEIN ENGINEERING, DESIGN & SELECTION, vol. 24, no. 5, 2011, pages 419 - 428
CURRENT OPINION IN CHEMICAL BIOLOGY, vol. 15, 2011, pages 789 - 797
PROC. NATL. ACAD. SCI. USA, vol. 105, no. 18, 2008, pages 6578 - 6583
SHEN L; GAO G; ZHANG Y; ZHANG H; YE Z; HUANG S; HUANG J; KANG J: "A single amino acid substitution confers enhanced methylation activity of mammalian Dnmt3b on chromatin DNA", NUCLEIC ACIDS RES., vol. 38, 2010, pages 6054 - 6064
See also references of EP 3382018A4
Attorney, Agent or Firm:
KAWAGUCHI, Yoshiyuki et al. (JP)
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