Title:
DNA ORIGAMI-BASED TUMOR NEOANTIGEN DELIVERY SYSTEM, PREPARATION METHOD THEREFOR, AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/087491
Kind Code:
A1
Abstract:
A preparation method for a DNA origami-based tumor neoantigen delivery system, comprising: selecting eight single-stranded DNAs, wherein 20 nt ployT structures extend from the 5' ends of three of the single-stranded DNAs and are used for pairing with tumor neoantigen peptide-nucleic acid reversible covalent coupling molecules containing sulfonium salt centers to realize the assembly of an OVA antigen and DNA origami; selecting another three single-stranded DNAs and extending CpG sequences at the 5' ends of the three single-stranded DNAs as immune activation co-factors to cooperate with an immune system for activation; mixing the single-stranded DNAs for preparing the DNA origami and the neoantigen peptide-nucleic acid reversible covalent coupling molecules; and carrying out high-temperature denaturation and annealing hybridization to form a triangular pyramid-shaped DNA tetrahedral nanocomposite, namely a DNA origami-based tumor neoantigen delivery system. Experiments prove that the constructed DNA origami-based delivery system containing sulfonium salt centers significantly inhibits tumor growth and lung metastasis of tumor cells.
Inventors:
LI ZIGANG (CN)
YIN FENG (CN)
ZHANG YAPING (CN)
JIANG LEYIN (CN)
LIU ZHAODI (CN)
YIN FENG (CN)
ZHANG YAPING (CN)
JIANG LEYIN (CN)
LIU ZHAODI (CN)
Application Number:
PCT/CN2021/141412
Publication Date:
May 25, 2023
Filing Date:
December 25, 2021
Export Citation:
Assignee:
SHENZHEN BAY LABORATORY PINGSHAN TRANSLATIONAL MEDICINE CENTER (CN)
UNIV PEKING SHENZHEN GRADUATE SCHOOL (CN)
UNIV PEKING SHENZHEN GRADUATE SCHOOL (CN)
International Classes:
A61K39/00; A61K39/385; A61K39/39; A61K47/54; C07K1/02; C12N9/02
Domestic Patent References:
| WO2021021523A1 | 2021-02-04 | |||
| WO2013052015A1 | 2013-04-11 |
Foreign References:
| CN112933219A | 2021-06-11 | |||
| CN111781186A | 2020-10-16 | |||
| CN110917121A | 2020-03-27 | |||
| CN109224080A | 2019-01-18 | |||
| CN107929751A | 2018-04-20 | |||
| CN108721634A | 2018-11-02 | |||
| US20150004193A1 | 2015-01-01 |
Other References:
HU YUE, CHEN ZHOU, ZHANG HE, LI MINGKAI, HOU ZHENG, LUO XIAOXING, XUE XIAOYAN: "Development of DNA tetrahedron-based drug delivery system", DRUG DELIVERY, ACADEMIC PRESS, ORLANDO, FL., US, vol. 24, no. 1, 1 January 2017 (2017-01-01), US , pages 1295 - 1301, XP093066495, ISSN: 1071-7544, DOI: 10.1080/10717544.2017.1373166
CHI, QINGJIA ET AL.: "DNA Nanostructure as an Efficient Drug Delivery Platform for Immunotherapy.", FRONTIERS IN PHARMACOLOGY., vol. 10, 28 January 2020 (2020-01-28), XP055851438, DOI: 10.3389/fphar.2019.01585
CHI, QINGJIA ET AL.: "DNA Nanostructure as an Efficient Drug Delivery Platform for Immunotherapy.", FRONTIERS IN PHARMACOLOGY., vol. 10, 28 January 2020 (2020-01-28), XP055851438, DOI: 10.3389/fphar.2019.01585
Attorney, Agent or Firm:
SHANGHAI XIAOXIANG INTELLECTUALPROPERTY AGENCY CO. (CN)
Download PDF:
Previous Patent: DIRECT-CONNECTION TYPE AIR COMPRESSOR
Next Patent: MODULAR SUBMERSIBLE GEAR PUMP AND SOAP DISPENSER
Next Patent: MODULAR SUBMERSIBLE GEAR PUMP AND SOAP DISPENSER