Title:
DNA PLASMIDS FOR THE FAST GENERATION OF HOMOLOGOUS RECOMBINATION VECTORS FOR CELL LINE DEVELOPMENT
Document Type and Number:
WIPO Patent Application WO/2018/144087
Kind Code:
A3
Abstract:
The present invention provides homologous recombination vectors to insert transgenic DNA in cells. These vectors shorten the production time and allow for easy generation of genetically modified cells. The invention allows the user to test multiple tags and to generate homozygous modified cell line using the homologous recombination vector. The invention can be used to generate knockout cells, to generate cell lines with knockin genes, to generate cell lines for drug screening against any target, to create transgenic animals, or in gene therapy.
Inventors:
PEREZ-LEAL OSCAR (CO)
Application Number:
PCT/US2017/059816
Publication Date:
October 25, 2018
Filing Date:
November 03, 2017
Export Citation:
Assignee:
UNIV TEMPLE (US)
International Classes:
C12N15/10; A01K67/027; C12N15/70; C12N15/81; C12N15/85
Foreign References:
| US20160186168A1 | 2016-06-30 | |||
| US20020004242A1 | 2002-01-10 | |||
| US20090111099A1 | 2009-04-30 | |||
| US20130117870A1 | 2013-05-09 | |||
| US20110033920A1 | 2011-02-10 |
Other References:
KIMURA ET AL.: "CRISPR/Cas9-mediated Reporter Knock-in in Mouse Haploid Embryonic Stem Cells", SCIENTIFIC REPORTS, vol. 5, no. 10710, 2015, pages 1 - 11, XP055554155
DATABASE GenBank [O] 10 June 2005 (2005-06-10), "Cloning vector pEX18ApGW", XP055554165, Database accession no. AY928469.1
DATABASE GenBank [O] 10 June 2005 (2005-06-10), "Cloning vector pEX18ApGW", XP055554165, Database accession no. AY928469.1
Attorney, Agent or Firm:
FONVILLE, Natalie et al. (US)
Download PDF: