Title:
DNA PRODUCTION METHOD AND DNA FRAGMENT JOINING KIT
Document Type and Number:
WIPO Patent Application WO/2019/009361
Kind Code:
A1
Abstract:
The present invention provides: a method that makes it possible to produce straight-chain or annular DNA by the joining of two or more types of DNA fragments at regions that have homologous base sequences; and a DNA fragment joining kit that uses said method. The present invention is a DNA production method that involves: preparing a reaction solution that includes two or more types of DNA fragments and a protein that acts as a RecA-family recombination enzyme; and producing straight-chain or annular DNA in the reaction solution by causing the two or more types of DNA fragments to be joined at regions that have homologous base sequences or regions that have complementary base sequences.
Inventors:
SUETSUGU MASAYUKI (JP)
KURATA TATSUAKI (JP)
KURATA TATSUAKI (JP)
Application Number:
PCT/JP2018/025528
Publication Date:
January 10, 2019
Filing Date:
July 05, 2018
Export Citation:
Assignee:
JAPAN SCIENCE & TECH AGENCY (JP)
International Classes:
C12N15/10
Domestic Patent References:
| WO2016080424A1 | 2016-05-26 | |||
| WO2016013592A1 | 2016-01-28 |
Foreign References:
| US20070037196A1 | 2007-02-15 | |||
| JP2016077180A | 2016-05-16 | |||
| JP2017132084A | 2017-08-03 | |||
| JP2017231732A | 2017-12-01 | |||
| US7575860B2 | 2009-08-18 | |||
| US7776532B2 | 2010-08-17 | |||
| US8968999B2 | 2015-03-03 | |||
| JP2016077180A | 2016-05-16 |
Other References:
BLEUIT ET AL., PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 98, no. 15, 2001, pages 8298 - 8305
KAGUNI JMKORNBERG A, CELL, vol. 38, 1984, pages 183 - 90
PENG HMARIANS KJ, PNAS, vol. 90, 1993, pages 8571 - 8575
See also references of EP 3650543A4
KAGUNI JMKORNBERG A, CELL, vol. 38, 1984, pages 183 - 90
PENG HMARIANS KJ, PNAS, vol. 90, 1993, pages 8571 - 8575
See also references of EP 3650543A4
Attorney, Agent or Firm:
MATSUNUMA Yasushi et al. (JP)
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