Title:
DNA SEQUENCE FROM KLUYVEROMYCES MARXIANUS COMPRISING REGULATORY REGIONS FOR EXPRESSING PROTEINS
Document Type and Number:
WIPO Patent Application WO2002070713
Kind Code:
A3
Abstract:
The invention relates to a DNA sequence comprising the triosephosphate isomerase gene including its regulatory sequences, to a DNA sequence, which is an active promoter, to expression and secretion systems containing said promoter sequence and to the use thereof and a method for producing proteins.
Inventors:
DOEHNER LEOPOLD (DE)
BECHER DIETMAR (DE)
SALIM SALAH (DE)
SIEKSTELE RIMANTAS (LT)
SASNAUSKAS KESTUTIS (LT)
BECHER DIETMAR (DE)
SALIM SALAH (DE)
SIEKSTELE RIMANTAS (LT)
SASNAUSKAS KESTUTIS (LT)
Application Number:
PCT/EP2002/000400
Publication Date:
March 13, 2003
Filing Date:
January 16, 2002
Export Citation:
Assignee:
TAD PHARMA GMBH (DE)
DOEHNER LEOPOLD (DE)
BECHER DIETMAR (DE)
SALIM SALAH (DE)
SIEKSTELE RIMANTAS (LT)
SASNAUSKAS KESTUTIS (LT)
DOEHNER LEOPOLD (DE)
BECHER DIETMAR (DE)
SALIM SALAH (DE)
SIEKSTELE RIMANTAS (LT)
SASNAUSKAS KESTUTIS (LT)
International Classes:
C12N15/09; C12N1/19; C12N9/90; C12N15/61; C12N15/81; C12P21/02; (IPC1-7): C12N15/61; C12N9/90; C12N15/62
Foreign References:
| FR2635115A1 | 1990-02-09 | |||
| EP0268772A2 | 1988-06-01 | |||
| EP0103409A2 | 1984-03-21 |
Other References:
COMPAGNO CONCETTA ET AL: "Isolation, nucleotide sequence, and physiological relevance of the gene encoding triose phosphate isomerase from Kluyveromyces lactis.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 65, no. 9, 1999, pages 4216 - 4219, XP002222527, ISSN: 0099-2240
BARTKEVICIUTE D ET AL: "HETEROLOGOUS EXPRESSION OF THE KLUYVEROMYCES MARXIANUS ENDOPOLYGALACTURONASE GENE (EPG1) USING VERSATILE AUTONOMOUSLY REPLICATING VECTOR FOR A WIDE RANGE OF HOST", ENZYME AND MICROBIAL TECHNOLOGY, STONEHAM, MA, US, vol. 26, June 2000 (2000-06-01), pages 653 - 656, XP000981517, ISSN: 0141-0229
SIEKSTELE R ET AL: "CLONING, TARGETED DISRUPTION AND HETEROLOGOUS EXPRESSION OF THE KLUYVEROMYCES MARXIANUS ENDOPOLYGALACTURONASE GENE (EPG1)", YEAST, CHICHESTER, SUSSEX, GB, vol. 15, no. 4, 15 March 1999 (1999-03-15), pages 311 - 322, XP000981653, ISSN: 0749-503X
SUMMERS R G ET AL: "ILLICIT SECRETION OF A CYTOPLASMIC PROTEIN INTO THE PERIPLASM OF ESCHERICHIA-COLI REQUIRES A SIGNAL PEPTIDE PLUS A PORTION OF THE COGNATE SECRETED PROTEIN DEMARCATION OF THE CRITICAL REGION OF THE MATURE PROTEIN", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 264, no. 33, 1989, pages 20074 - 20081, XP002222528, ISSN: 0021-9258
BARTKEVICIUTE D ET AL: "HETEROLOGOUS EXPRESSION OF THE KLUYVEROMYCES MARXIANUS ENDOPOLYGALACTURONASE GENE (EPG1) USING VERSATILE AUTONOMOUSLY REPLICATING VECTOR FOR A WIDE RANGE OF HOST", ENZYME AND MICROBIAL TECHNOLOGY, STONEHAM, MA, US, vol. 26, June 2000 (2000-06-01), pages 653 - 656, XP000981517, ISSN: 0141-0229
SIEKSTELE R ET AL: "CLONING, TARGETED DISRUPTION AND HETEROLOGOUS EXPRESSION OF THE KLUYVEROMYCES MARXIANUS ENDOPOLYGALACTURONASE GENE (EPG1)", YEAST, CHICHESTER, SUSSEX, GB, vol. 15, no. 4, 15 March 1999 (1999-03-15), pages 311 - 322, XP000981653, ISSN: 0749-503X
SUMMERS R G ET AL: "ILLICIT SECRETION OF A CYTOPLASMIC PROTEIN INTO THE PERIPLASM OF ESCHERICHIA-COLI REQUIRES A SIGNAL PEPTIDE PLUS A PORTION OF THE COGNATE SECRETED PROTEIN DEMARCATION OF THE CRITICAL REGION OF THE MATURE PROTEIN", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 264, no. 33, 1989, pages 20074 - 20081, XP002222528, ISSN: 0021-9258
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