Title:
DNA SEQUENCING ENZYMES
Document Type and Number:
WIPO Patent Application WO1995004162
Kind Code:
A3
Abstract:
There are provided variant family B DNA polymerases having no 3'->5' exonuclease activity. These variant polymerases have utility as DNA sequencing polymerases. Methods for DNA sequencing with family B DNA polymerases and chain-terminating nucleotides not previously used for sequencing have been developed. The methods disclosed involve the use of family B DNA polymerases not known heretofore to have utility in DNA sequencing, such as variant or wild type forms of phage T4 DNA polymerase or Escherichia coli DNA polymerase II, with novel combinations of deoxynucleotides and chain-terminating nucleotides.
Inventors:
GOODMAN MYRON F
REHA-KRANTZ LINDA J
REHA-KRANTZ LINDA J
Application Number:
PCT/US1994/008610
Publication Date:
June 01, 1995
Filing Date:
August 01, 1994
Export Citation:
Assignee:
UNIV SOUTHERN CALIFORNIA (US)
UNIV ALBERTA (CA)
UNIV ALBERTA (CA)
International Classes:
C07H19/04; C07H21/00; C12N9/12; C12N15/09; C12Q1/68; C12Q1/6869; C12R1/19; (IPC1-7): C12Q1/68; C07H21/00; C12N9/12
Domestic Patent References:
| WO1991016446A1 | 1991-10-31 | |||
| WO1994005684A1 | 1994-03-17 | |||
| WO1991006679A1 | 1991-05-16 |
Foreign References:
| EP0516245A1 | 1992-12-02 | |||
| EP0309969A2 | 1989-04-05 | |||
| DE3546374A1 | 1987-07-02 | |||
| DE4214112A1 | 1993-02-04 | |||
| EP0252683A2 | 1988-01-13 | |||
| US4666892A | 1987-05-19 |
Other References:
BERNAD, A. ET AL.: "A conserved 3'-5' Exonuclease active site in prokaryotic and eurokaryotic DNA polymerases", CELL, vol. 59, October 1989 (1989-10-01), NEW YORK US, pages 219 - 228
ITO,J. ET AL.: "compliation and aligment of dna polymerase sequences", NUCLEIC ACIDS RESEARCH., vol. 19, no. 15, 1991, ARLINGTON, VIRGINIA US, pages 4045 - 57
ITO,J. ET AL.: "compliation and aligment of dna polymerase sequences", NUCLEIC ACIDS RESEARCH., vol. 19, no. 15, 1991, ARLINGTON, VIRGINIA US, pages 4045 - 57
Download PDF: