Title:
DNA VACCINE, METHOD OF INDUCING THE IMMUNE RESPONSE, METHOD OF IMMUNISATION, ANTIBODIES SPECIFICALLY RECOGNISING THE H5 HAEMAGGLUTININ OF AN INFLUENZA VIRUS AND USE OF THE DNA VACCINE
Document Type and Number:
WIPO Patent Application WO/2013/043067
Kind Code:
A3
Abstract:
The object of the invention is a DNA vaccine, method of inducing the immune response, antibodies specifically recognising the haemagglutinin H5 of an influenza virus and application of the DNA vaccine. According to the invention, one or two-fold immunisation of hens with DNA vaccine containing a cDNA encoding the modified H5 haemagglutinin HA protein, i.e. with the deletion of the cleavage site between HA subunits (this provides for greater safety of the vaccines). Moreover, the encoding region of the HA is modified in such a way that protein production in the bird cells should achieve maximal yield. The main modification is codon optimisation for the hens and deletion of the site of proteolytic cleavage between subunits HA1 and HA2.
Inventors:
SIRKO AGNIESZKA (PL)
GORA-SOCHACKA ANNA (PL)
ZAGORSKI-OSTOJA WLODZIMIERZ (PL)
STACHYRA ANNA (PL)
SAWICKA ROZA (PL)
SZEWCZYK BOGUSLAW (PL)
GROMADZKA BEATA (PL)
SACZYNSKA VIOLETTA (PL)
FLORYS KATARZYNA (PL)
MINTA ZENON (PL)
SMIETANKA KRZYSZTOF (PL)
GORA-SOCHACKA ANNA (PL)
ZAGORSKI-OSTOJA WLODZIMIERZ (PL)
STACHYRA ANNA (PL)
SAWICKA ROZA (PL)
SZEWCZYK BOGUSLAW (PL)
GROMADZKA BEATA (PL)
SACZYNSKA VIOLETTA (PL)
FLORYS KATARZYNA (PL)
MINTA ZENON (PL)
SMIETANKA KRZYSZTOF (PL)
Application Number:
PCT/PL2012/000095
Publication Date:
November 14, 2013
Filing Date:
September 21, 2012
Export Citation:
Assignee:
INST BIOCHEMII I BIOFIZYKI PAN (PL)
International Classes:
A61K39/145
Domestic Patent References:
| WO2009092038A1 | 2009-07-23 | |||
| WO2010115133A2 | 2010-10-07 |
Foreign References:
| EP2284260A1 | 2011-02-16 |
Other References:
RAO SRINIVAS ET AL: "Multivalent HA DNA Vaccination Protects against Highly Pathogenic H5N1 Avian Influenza Infection in Chickens and Mice", PLOS ONE, PUBLIC LIBRARY OF SCIENCE, US, vol. 3, no. 6, 1 June 2008 (2008-06-01), pages E2432 - 1, XP002527215, ISSN: 1932-6203, DOI: 10.1371/JOURNAL.PONE.0002432
JIANG ET AL: "Enhanced protective efficacy of H5 subtype avian influenza DNA vaccine with codon optimized HA gene in a pCAGGS plasmid vector", ANTIVIRAL RESEARCH, ELSEVIER BV, NL, vol. 75, no. 3, 13 June 2007 (2007-06-13), pages 234 - 241, XP022114363, ISSN: 0166-3542, DOI: 10.1016/J.ANTIVIRAL.2007.03.009
TORO H ET AL: "Protection of chickens against avian influenza with nonreplicating adenovirus-vectored vaccine", POULTRY SCIENCE, CHAMPAIGN, IL, US, vol. 88, no. 4, 1 April 2009 (2009-04-01), pages 867 - 871, XP008119748, ISSN: 0032-5791, DOI: 10.3382/PS.2008-00333
HENKE ANDREAS ET AL: "Co-expression of interleukin-2 by a bicistronic plasmid increases the efficacy of DNA immunization to prevent influenza virus infections.", INTERVIROLOGY 2006, vol. 49, no. 4, 2006, pages 249 - 252, XP002712860, ISSN: 0300-5526
JEONG HO PARK ET AL: "Protection of chicken against very virulent IBDV provided by in ovo priming with DNA vaccine and boosting with killed vaccine and the adjuvant effects of plasmid-encoded chicken interleukin-2 and interferon-[gamma]", JOURNAL OF VETERINARY SCIENCE, vol. 10, no. 2, 1 January 2009 (2009-01-01), pages 131, XP055079018, ISSN: 1229-845X, DOI: 10.4142/jvs.2009.10.2.131
SONG J M ET AL: "Protective immunity against H5N1 influenza virus by a single dose vaccination with virus-like particles", VIROLOGY, ELSEVIER, AMSTERDAM, NL, vol. 405, no. 1, 15 September 2010 (2010-09-15), pages 165 - 175, XP027180037, ISSN: 0042-6822, [retrieved on 20100728]
YIXIN CHEN ET AL: "Broad Cross-Protection against H5N1 Avian Influenza Virus Infection by Means of Monoclonal Antibodies that Map to Conserved Viral Epitopes", THE JOURNAL OF INFECTIOUS DISEASES, vol. 199, no. 1, 1 January 2009 (2009-01-01), pages 49 - 58, XP055023361, ISSN: 0022-1899, DOI: 10.1086/594374
DATABASE EMBL [online] 1 July 2002 (2002-07-01), "Gallus gallus breed broiler interleukin-2 precursor (IL2) mRNA, complete cds.", XP002712861, retrieved from EBI accession no. EM_STD:AF483599 Database accession no. AF483599
DATABASE EMBL [online] 31 March 2007 (2007-03-31), "Influenza A virus (A/whooper swan/Germany/R88/06(H5N1)) HA gene for hemagglutinin, genomic RNA", XP002712862, retrieved from EBI accession no. EM_STD:AM403462 Database accession no. AM403462
JIANG ET AL: "Enhanced protective efficacy of H5 subtype avian influenza DNA vaccine with codon optimized HA gene in a pCAGGS plasmid vector", ANTIVIRAL RESEARCH, ELSEVIER BV, NL, vol. 75, no. 3, 13 June 2007 (2007-06-13), pages 234 - 241, XP022114363, ISSN: 0166-3542, DOI: 10.1016/J.ANTIVIRAL.2007.03.009
TORO H ET AL: "Protection of chickens against avian influenza with nonreplicating adenovirus-vectored vaccine", POULTRY SCIENCE, CHAMPAIGN, IL, US, vol. 88, no. 4, 1 April 2009 (2009-04-01), pages 867 - 871, XP008119748, ISSN: 0032-5791, DOI: 10.3382/PS.2008-00333
HENKE ANDREAS ET AL: "Co-expression of interleukin-2 by a bicistronic plasmid increases the efficacy of DNA immunization to prevent influenza virus infections.", INTERVIROLOGY 2006, vol. 49, no. 4, 2006, pages 249 - 252, XP002712860, ISSN: 0300-5526
JEONG HO PARK ET AL: "Protection of chicken against very virulent IBDV provided by in ovo priming with DNA vaccine and boosting with killed vaccine and the adjuvant effects of plasmid-encoded chicken interleukin-2 and interferon-[gamma]", JOURNAL OF VETERINARY SCIENCE, vol. 10, no. 2, 1 January 2009 (2009-01-01), pages 131, XP055079018, ISSN: 1229-845X, DOI: 10.4142/jvs.2009.10.2.131
SONG J M ET AL: "Protective immunity against H5N1 influenza virus by a single dose vaccination with virus-like particles", VIROLOGY, ELSEVIER, AMSTERDAM, NL, vol. 405, no. 1, 15 September 2010 (2010-09-15), pages 165 - 175, XP027180037, ISSN: 0042-6822, [retrieved on 20100728]
YIXIN CHEN ET AL: "Broad Cross-Protection against H5N1 Avian Influenza Virus Infection by Means of Monoclonal Antibodies that Map to Conserved Viral Epitopes", THE JOURNAL OF INFECTIOUS DISEASES, vol. 199, no. 1, 1 January 2009 (2009-01-01), pages 49 - 58, XP055023361, ISSN: 0022-1899, DOI: 10.1086/594374
DATABASE EMBL [online] 1 July 2002 (2002-07-01), "Gallus gallus breed broiler interleukin-2 precursor (IL2) mRNA, complete cds.", XP002712861, retrieved from EBI accession no. EM_STD:AF483599 Database accession no. AF483599
DATABASE EMBL [online] 31 March 2007 (2007-03-31), "Influenza A virus (A/whooper swan/Germany/R88/06(H5N1)) HA gene for hemagglutinin, genomic RNA", XP002712862, retrieved from EBI accession no. EM_STD:AM403462 Database accession no. AM403462
Attorney, Agent or Firm:
TWARDOWSKA, Aleksandra (ul. Nowogrodzka 47a, Warszawa, PL)
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