Title:
DNA VECTORS, TRANSPOSONS AND TRANSPOSASES FOR EUKARYOTIC GENOME MODIFICATION
Document Type and Number:
WIPO Patent Application WO/2017/062668
Kind Code:
A3
Abstract:
The present invention provides polynucleotide vectors for high expression of heterologous genes. Some vectors further comprise novel transposons and transposases that further improve expression. Further disclosed are vectors that can be used in a gene transfer system for stably introducing nucleic acids into the DNA of a cell. The gene transfer systems can be used in methods, for example, gene expression, bioprocessing, gene therapy, insertional mutagenesis, or gene discovery.
Inventors:
MINSHULL JEREMY (US)
WELCH MARK (US)
GOVINDRAJAN SRIDHAR (US)
LEE MAGGIE (US)
CAVES KATE (US)
NESS JON (US)
WELCH MARK (US)
GOVINDRAJAN SRIDHAR (US)
LEE MAGGIE (US)
CAVES KATE (US)
NESS JON (US)
Application Number:
PCT/US2016/055824
Publication Date:
August 03, 2017
Filing Date:
October 06, 2016
Export Citation:
Assignee:
DNA2 0 INC (US)
International Classes:
C12N15/74; A61K31/713; C12N1/19; C12N15/11; C12N15/67; C12N15/85; C12N15/867; C12N15/87; C12N15/90; C12Q1/68
Domestic Patent References:
| WO2014125382A2 | 2014-08-21 |
Foreign References:
| US20100146655A1 | 2010-06-10 | |||
| US20040203158A1 | 2004-10-14 | |||
| US20140178914A1 | 2014-06-26 | |||
| US20040110295A1 | 2004-06-10 | |||
| US20150232836A1 | 2015-08-20 | |||
| US20150232862A1 | 2015-08-20 | |||
| US20090186020A1 | 2009-07-23 | |||
| US6410314B1 | 2002-06-25 | |||
| US20100138939A1 | 2010-06-03 | |||
| US20130045539A1 | 2013-02-21 | |||
| US20120225034A1 | 2012-09-06 | |||
| US20120087862A1 | 2012-04-12 |
Other References:
HIKOSAKA, A ET AL.: "Evolution of the Xenopus piggyBac Transposon Family TxpB: Domesticated and Untamed Strategies of Transposon Subfamilies", MOLECULAR BIOLOGY AND EVOLUTION, vol. 24, no. 12, 13 October 2007 (2007-10-13), pages 2648 - 2656, XP055242774
HELLSTEN, U ET AL.: "The Genome of the Western Clawed Frog Xenopus tropicalis", SCIENCE, vol. 328, no. 5978, 30 April 2010 (2010-04-30), pages 633 - 636, XP055065176
AKHTAR, W ET AL.: "Chromatin Position Effects Assayed by Thousands of Reporters Integrated in Parallel", CELL, vol. 154, 15 August 2013 (2013-08-15), pages 914 - 927, XP055407898
LIANG, Z ET AL.: "Phylogenetic Analysis of the Species Theilovirus: Emerging Murine and Human Pathogens", JOURNAL OF VIROLOGY, vol. 82, no. 23, December 2008 (2008-12-01), pages 11545 - 11554, XP055407900
WANG, J ET AL.: "Large diversity of the piggyBac-like elements in the genome of Tribolium castaneum", INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, vol. 38, no. 4, April 2008 (2008-04-01), pages 490 - 498, XP022520478
OKAMOTO, H ET AL.: "Typing Hepatitis B Virus by Homology in Nucleotide Sequence: Comparison of Surface Antigen Subtypes", JOURNAL OF GENERAL VIROLOGY, vol. 69, October 1998 (1998-10-01), pages 2575 - 2583, XP000672123
TAS, H ET AL.: "An Integrated System for Precise Genome Modification in Escherichia coli", PLOS ONE, vol. 10, no. 9, 2 September 2015 (2015-09-02), pages 1 - 19, XP055407909
CABIANCA, DS ET AL.: "A Long ncRNA Links Copy Number Variation to a Polycomb/Trithorax Epigenetic Switch in FSHD Muscular Dystrophy", CELL, vol. 149, no. 4, 11 May 2012 (2012-05-11), pages 819 - 831, XP028485961
YAN, G ET AL.: "Genome sequencing and comparison of two nonhuman primate animal models , the cynomolgus and Chinese rhesus macaques", NATURE BIOTECHNOLOGY, vol. 29, no. 11, 16 October 2011 (2011-10-16), pages 1019 - 1023, XP055126993
HELLSTEN, U ET AL.: "The Genome of the Western Clawed Frog Xenopus tropicalis", SCIENCE, vol. 328, no. 5978, 30 April 2010 (2010-04-30), pages 633 - 636, XP055065176
AKHTAR, W ET AL.: "Chromatin Position Effects Assayed by Thousands of Reporters Integrated in Parallel", CELL, vol. 154, 15 August 2013 (2013-08-15), pages 914 - 927, XP055407898
LIANG, Z ET AL.: "Phylogenetic Analysis of the Species Theilovirus: Emerging Murine and Human Pathogens", JOURNAL OF VIROLOGY, vol. 82, no. 23, December 2008 (2008-12-01), pages 11545 - 11554, XP055407900
WANG, J ET AL.: "Large diversity of the piggyBac-like elements in the genome of Tribolium castaneum", INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, vol. 38, no. 4, April 2008 (2008-04-01), pages 490 - 498, XP022520478
OKAMOTO, H ET AL.: "Typing Hepatitis B Virus by Homology in Nucleotide Sequence: Comparison of Surface Antigen Subtypes", JOURNAL OF GENERAL VIROLOGY, vol. 69, October 1998 (1998-10-01), pages 2575 - 2583, XP000672123
TAS, H ET AL.: "An Integrated System for Precise Genome Modification in Escherichia coli", PLOS ONE, vol. 10, no. 9, 2 September 2015 (2015-09-02), pages 1 - 19, XP055407909
CABIANCA, DS ET AL.: "A Long ncRNA Links Copy Number Variation to a Polycomb/Trithorax Epigenetic Switch in FSHD Muscular Dystrophy", CELL, vol. 149, no. 4, 11 May 2012 (2012-05-11), pages 819 - 831, XP028485961
YAN, G ET AL.: "Genome sequencing and comparison of two nonhuman primate animal models , the cynomolgus and Chinese rhesus macaques", NATURE BIOTECHNOLOGY, vol. 29, no. 11, 16 October 2011 (2011-10-16), pages 1019 - 1023, XP055126993
Attorney, Agent or Firm:
LIEBESCHUETZ, Joe et al. (US)
Download PDF: