Title:
DONKEY-DERIVED CHARACTERISTIC PEPTIDE OF DONKEY-HIDE GELATIN AND PRODUCTS THEREOF, AND METHOD FOR IDENTIFYING DONKEY-HIDE GELATIN AND PRODUCTS THEREOF
Document Type and Number:
WIPO Patent Application WO/2021/003926
Kind Code:
A1
Abstract:
A donkey-derived characteristic peptide for identifying donkey-hide gelatin and products thereof, the peptide fragment comprising a first peptide fragment: HGNRGEPGPVGSVGPVGAVGPRGPSGPQGVRGDK and/or a second peptide fragment: GPTGEPGK. Two donkey-derived characteristic peptide fragments are screened out and obtained by means of a large number of experiments. The two peptide fragments have high specificity, and may be used to identify donkey hide, donkey-hide gelatin and donkey-hide gelatin products. Also provided is a method for identifying donkey-hide gelatin and products thereof, wherein optimal chromatographic conditions and mass spectrometry conditions are screened out by means of a large number of experiments. The entire method is simple to operate and has high accuracy, is beneficial to the quality control of donkey-hide gelatin, and may greatly enhance the efficiency and accuracy of detecting donkey-hide gelatin and products thereof, and is of great significance to ensuring the quality of donkey-hide gelatin and products thereof.
Inventors:
LIU RUI (CN)
CAI SHUO (CN)
ZHAO KEXUAN (CN)
JIANG MENGTONG (CN)
ZHENG YUNFENG (CN)
DUAN JINAO (CN)
CAI SHUO (CN)
ZHAO KEXUAN (CN)
JIANG MENGTONG (CN)
ZHENG YUNFENG (CN)
DUAN JINAO (CN)
Application Number:
PCT/CN2019/117384
Publication Date:
January 14, 2021
Filing Date:
November 12, 2019
Export Citation:
Assignee:
UNIV NANJING CHINESE MEDICINE (CN)
International Classes:
G01N30/88
Foreign References:
| CN105301165A | 2016-02-03 | |||
| CN109187835A | 2019-01-11 | |||
| CN106841437A | 2017-06-13 | |||
| CN109142598A | 2019-01-04 |
Other References:
LI XUE, SHI FENG, GONG LIPING, HANG BAOJIAN, LI DAOYUAN, CHI LIANLI: "Species-specific identification of collagen components in Colla corii asini using a nano-liquid chromatography tandem mass spectrometry proteomics approach", INTERNATIONAL JOURNAL OF NANOMEDICINE, vol. 12, 1 January 2017 (2017-01-01), pages 4443 - 4454, XP055772675, DOI: 10.2147/IJN.S136819
Attorney, Agent or Firm:
NANJING JINGWEI PATENT & TRADEMARK AGENCY CO., LTD (CN)
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