DOXA PROTEIN MUTANT, AND CODING GENE AND APPLICATIONS THEREOF

Title:

DOXA PROTEIN MUTANT, AND CODING GENE AND APPLICATIONS THEREOF

Document Type and Number:

WIPO Patent Application WO/2017/198085

Kind Code:

A1

Abstract:

A DoxA protein mutant represented by an amino acid sequence such as SEQ ID No.16, and a coding gene thereof. The protein mutant or the coding gene thereof can be used for producing doxorubicin. Streptomyces capable of efficiently expressing epirubicin. A dnmV gene of an original strain is replaced by an avrE gene in situ, the doxA gene is mutated into a gene of the protein represented by the code SEQ ID No.16 in construction, and the potency of the epirubicin of fermentation broth reaches 102.0 μg/ml.

Inventors:

ZHOU MIN (CN)
JIANG XING (CN)
HAN SHUANG (CN)
HE YINGYING (CN)
ZHENG LINGHUI (CN)

Application Number:

PCT/CN2017/083485

Publication Date:

November 23, 2017

Filing Date:

May 08, 2017

Export Citation:

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Assignee:

ZHEJIANG HISUN PHARM CO LTD (CN)

International Classes:

C12N1/21; C12N9/02; C12N15/53; C12N15/76; C12P19/56; C12R1/465

Foreign References:

CN105861455A	2016-08-17
CN104059892A	2014-09-24
CN102363755A	2012-02-29
CN101892186A	2010-11-24
CN101802168A	2010-08-11

Other References:

DATABASE GenBank [O] 13 January 1999 (1999-01-13), LOMOVSKAYA, N. ET AL.: "daunorubicin C-14 hydroxylase [Streptomyces peucetius", XP055442071, retrieved from NCBI Database accession no. AAD04715.1
MADDURI, K. ET AL.: "Production of the antitumor drug epirubicin (4'-epi-doxorubincin) and its precursor by a genetically engineered strain of Streptomyces peucetius", NATURE BIOTECHNOLOGY, vol. 16, no. 1, 31 January 1998 (1998-01-31), pages 69 - 74, XP002210929
See also references of EP 3460055A4