Title:
DROPLET MICROFLUIDIC DEVICE AND METHODS OF SENSING THE RESULT OF AN ASSAY THEREIN
Document Type and Number:
WIPO Patent Application WO/2017/038063
Kind Code:
A1
Abstract:
A method of determining the result of an assay in a microfluidic device includes the steps of: dispensing a sample droplet onto a first portion of an electrode array of the microfluidic device; dispensing a reagent droplet onto a second portion of the electrode array of the microfluidic device; controlling actuation voltages applied to the electrode array to mix the sample droplet and the reagent droplet into a product droplet; sensing a dynamic property of the product droplet; and determining an assay of the sample droplet based on the sensed dynamic property. The dynamic property is a physical property of the product droplet that influences a transport property of the product droplet on the electrode array. Example dynamic properties of the product droplet include the moveable state, split-able state, and viscosity based on droplet properties. The method may be used to perform an amoebocyte lysate (LAL) assay.
Inventors:
HADWEN BENJAMIN JAMES
JACOBS ADRIAN MARC SIMON
HECTOR JASON RODERICK
BROWNLOW MICHAEL JAMES
ADACHI MASAHIRO
SKINNER ALISON MARY
CHILDS MARK
JACOBS ADRIAN MARC SIMON
HECTOR JASON RODERICK
BROWNLOW MICHAEL JAMES
ADACHI MASAHIRO
SKINNER ALISON MARY
CHILDS MARK
Application Number:
PCT/JP2016/003881
Publication Date:
March 09, 2017
Filing Date:
August 26, 2016
Export Citation:
Assignee:
SHARP KK (JP)
International Classes:
G01N35/08; G01N1/00; G01N33/86; G01N37/00
Foreign References:
| US20070241068A1 | 2007-10-18 | |||
| US20110104725A1 | 2011-05-05 | |||
| US20050106742A1 | 2005-05-19 | |||
| US20100320088A1 | 2010-12-23 |
Other References:
NELSON, WYATT: "A Micro Extensional Filament Rheometer Enabled by EWOD", PROC. INT. CONF. MEMS, 24 January 2010 (2010-01-24), pages 75 - 78, XP031670917
LEDERER, THOMAS: "An Electrowetting on Dielectrics Based Lab-on a-Chip Utilizing an Integrated High Fundamental Frequency Quartz Crystal Resonator as a Biosensor", PROCEDIA ENGINEERING, vol. 5, 2010, pages 959 - 964, XP027483824
HONG, JIWOO: "Effects of Drop Size and Viscosity on Spreading Dynamics in DC Electrowetting", LANGMUIR, vol. 29, 2013, pages 9118 - 9125, XP055367562
See also references of EP 3341740A4
LEDERER, THOMAS: "An Electrowetting on Dielectrics Based Lab-on a-Chip Utilizing an Integrated High Fundamental Frequency Quartz Crystal Resonator as a Biosensor", PROCEDIA ENGINEERING, vol. 5, 2010, pages 959 - 964, XP027483824
HONG, JIWOO: "Effects of Drop Size and Viscosity on Spreading Dynamics in DC Electrowetting", LANGMUIR, vol. 29, 2013, pages 9118 - 9125, XP055367562
See also references of EP 3341740A4
Attorney, Agent or Firm:
HARAKENZO WORLD PATENT & TRADEMARK (JP)
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