Title:
DUAL-LUCIFERASE REPORTER GENE DETECTION SYSTEM AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2024/031306
Kind Code:
A1
Abstract:
Provided are a dual-luciferase reporter gene detection system and use thereof. The detection system comprises Gaussia luciferase, Pleuromamma xiphias luciferase, and a substrate, and the substrate is coelenterazine or a derivative thereof. Compared to other double-luciferase reporter gene detection systems, the dual-luciferase reporter gene detection system features higher signal intensities and simple reaction conditions.
Inventors:
ZHUO SHITIAN (CN)
TENG BO (CN)
ZHANG WENWEI (CN)
CHEN AO (CN)
XU XUN (CN)
TENG BO (CN)
ZHANG WENWEI (CN)
CHEN AO (CN)
XU XUN (CN)
Application Number:
PCT/CN2022/111058
Publication Date:
February 15, 2024
Filing Date:
August 09, 2022
Export Citation:
Assignee:
BGI SHENZHEN (CN)
International Classes:
C12Q1/66; C07D487/04; G01N21/64
Foreign References:
| CN112368397A | 2021-02-12 | |||
| CN114807299A | 2022-07-29 | |||
| CN101636503A | 2010-01-27 | |||
| CN101636504A | 2010-01-27 |
Other References:
HEISE, K ET AL.: "Dual Luciferase Assay for Secreted Luciferases Based on Gaussia and NanoLuc", ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES, vol. 11, no. 4, 31 May 2013 (2013-05-31), pages 244 - 252, XP055697314, DOI: 10.1089/adt.2013.509
COUTANT, E. P ET AL.: "Bioluminescence Profiling of NanoKAZ/NanoLuc Luciferase Using a Chemical Library of Coelenterazine Analogues", CHEMICAL BIOLOGY, vol. 26, 31 December 2020 (2020-12-31), pages 948 - 958, XP071851866, DOI: 10.1002/chem.201904844
TAKENAKA, Y. ET AL.: "Computational Analysis and Functional Expression of Ancestral Copepod Luciferase", GENE, vol. 528, 23 July 2013 (2013-07-23), pages 201 - 205, XP028700819, DOI: 10.1016/j.gene.2013.07.011
COUTANT, E. P ET AL.: "Bioluminescence Profiling of NanoKAZ/NanoLuc Luciferase Using a Chemical Library of Coelenterazine Analogues", CHEMICAL BIOLOGY, vol. 26, 31 December 2020 (2020-12-31), pages 948 - 958, XP071851866, DOI: 10.1002/chem.201904844
TAKENAKA, Y. ET AL.: "Computational Analysis and Functional Expression of Ancestral Copepod Luciferase", GENE, vol. 528, 23 July 2013 (2013-07-23), pages 201 - 205, XP028700819, DOI: 10.1016/j.gene.2013.07.011
Attorney, Agent or Firm:
UNITALEN ATTORNEYS AT LAW (CN)
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