DYNAMIC CHEMICAL IMAGING OF BIOLOGICAL CELLS AND OTHER SUBJECTS

Title:

DYNAMIC CHEMICAL IMAGING OF BIOLOGICAL CELLS AND OTHER SUBJECTS

Document Type and Number:

WIPO Patent Application WO2006005022

Kind Code:

A3

Abstract:

The invention relates to methods of dynamic chemical imaging, including methods of cellular imaging. The method comprises illuminating at least a portion of a cell with substantially monochromatic light and assessing Raman-shifted light scattered from the illuminated portion at a plurality of discrete times. The Raman-shifted light can be assessed at a plurality of Raman shift (RS) values at each of the discrete times, and the RS values can be selected to be characteristic of a pre-selected component at each of the discrete times. Multivariate analysis of Raman spectral features of the images thus obtained can yield the location and chemical identity of components in the field of view. This information can be combined or overlaid with other spectral data (e.g., a visible microscopic image) obtained from the field of view.

Inventors:

MAIER JOHN S (US)
TREADO PATRICK J (US)
VOIGT THOMAS C (US)
TUSCHEL DAVID (US)

Application Number:

PCT/US2005/023638

Publication Date:

June 15, 2006

Filing Date:

June 30, 2005

Export Citation:

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Assignee:

CHEMIMAGE CORP (US)
MAIER JOHN S (US)
TREADO PATRICK J (US)
VOIGT THOMAS C (US)
TUSCHEL DAVID (US)

International Classes:

G01N21/65; G01J3/44

Foreign References:

US20010044129A1	2001-11-22
US6002476A	1999-12-14

Other References: