Title:
DYNAMIC GENOME ENGINEERING
Document Type and Number:
WIPO Patent Application WO/2018/081535
Kind Code:
A3
Abstract:
Provided herein, in some embodiments, are genomic editing constructs that can achieve nearly 100% recombination efficiency within a select population of bacterial cells.
Inventors:
LU TIMOTHY (US)
FARZADFARD FAHIM (US)
FARZADFARD FAHIM (US)
Application Number:
PCT/US2017/058723
Publication Date:
June 07, 2018
Filing Date:
October 27, 2017
Export Citation:
Assignee:
MASSACHUSETTS INST TECHNOLOGY (US)
International Classes:
C12N15/10
Domestic Patent References:
| WO2016025719A1 | 2016-02-18 | |||
| WO2003104470A2 | 2003-12-18 |
Foreign References:
| US20140113375A1 | 2014-04-24 |
Other References:
F. FARZADFARD ET AL: "Genomically encoded analog memory with precise in vivo DNA writing in living cell populations", SCIENCE, vol. 346, no. 6211, 14 November 2014 (2014-11-14), pages 1256272 - 1256272, XP055256180, ISSN: 0036-8075, DOI: 10.1126/science.1256272
TRIANA N. DALIA ET AL: "Enhancing multiplex genome editing by natural transformation (MuGENT) via inactivation of ssDNA exonucleases", NUCLEIC ACIDS RESEARCH, vol. 45, no. 12, 7 July 2017 (2017-07-07), pages 7527 - 7537, XP055446202, ISSN: 0305-1048, DOI: 10.1093/nar/gkx496
TRIANA N. DALIA ET AL: "Enhancing multiplex genome editing by natural transformation (MuGENT) via inactivation of ssDNA exonucleases", NUCLEIC ACIDS RESEARCH, vol. 45, no. 12, 7 July 2017 (2017-07-07), pages 7527 - 7537, XP055446202, ISSN: 0305-1048, DOI: 10.1093/nar/gkx496
Attorney, Agent or Firm:
GE, Zhiyun (US)
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