Title:
EARLY RECOGNITION PROCESS OF HPV-ASSOCIATED CARCINEARLY RECOGNITION PROCESS OF HPV-ASSOCIATED CARCINOMAS OR HIGH-GRADE, HPV-CAUSED DYSPLASIAS OMAS OR HIGH-GRADE, HPV-CAUSED DYSPLASIAS
Document Type and Number:
WIPO Patent Application WO1996026293
Kind Code:
A3
Abstract:
PCT No. PCT/DE96/00306 Sec. 371 Date Jan. 28, 1998 Sec. 102(e) Date Jan. 28, 1998 PCT Filed Feb. 23, 1996 PCT Pub. No. WO96/26293 PCT Pub. Date Aug. 29, 1996This invention relates to a method of detection of an mRNA having HPV and cellular sequences in a body sample, comprising the following steps: (a) obtaining a body sample; (b) isolating mRNA from the body sample of (a); (c) translating the mRNA of (b) into cDNA using a primer common for reverse transcription; (d) amplifying the cDNA of (c) by a PCR reaction with a 5' HPV primer and a 3' primer having sequences of the primer of (c); (e) cleaving the amplified cDNA of (d) with an endonuclease cleaving on the 5' side of the HPV polyadenylation sequence; (f) amplifying the non-cleaved cDNA of (e) with the primers of (d) or with "nested" primers; and (g) detecting the amplified cDNA of (f). Furthermore, this invention concerns the use of such a method of early detection of HPV-associated carcinomas and extreme dysplasias caused by HPV, respectively.
Inventors:
VON KNEBEL-DOEBERITZ MAGNUS (DE)
WOERNER STEFAN (DE)
EMMERICH FLORIAN (DE)
WOERNER STEFAN (DE)
EMMERICH FLORIAN (DE)
Application Number:
PCT/DE1996/000306
Publication Date:
December 12, 1996
Filing Date:
February 23, 1996
Export Citation:
Assignee:
DEUTSCHES KREBSFORSCH (DE)
VON KNEBEL DOEBERITZ MAGNUS (DE)
WOERNER STEFAN (DE)
EMMERICH FLORIAN (DE)
VON KNEBEL DOEBERITZ MAGNUS (DE)
WOERNER STEFAN (DE)
EMMERICH FLORIAN (DE)
International Classes:
C12Q1/68; C12N15/09; C12Q1/686; C12Q1/70; (IPC1-7): C12Q1/70
Domestic Patent References:
| WO1994026934A2 | 1994-11-24 | |||
| WO1991008312A1 | 1991-06-13 | |||
| WO1988006634A1 | 1988-09-07 |
Foreign References:
| EP0373352A2 | 1990-06-20 | |||
| EP0402132A2 | 1990-12-12 | |||
| EP0466367A1 | 1992-01-15 |
Other References:
CORNELISSEN M T E ET AL: "UNIFORMITY OF THE SPLICING PATTERN OF THE E6/E7 TRANSCRIPTS IN HUMAN PAPILLOMAVIRUS TYPE 16-TRANSFORMED HUMAN FIBROBLASTS, HUMAN CERVICAL PREMALIGNANT LESIONS AND CARCINOMAS", JOURNAL OF GENERAL VIROLOGY, vol. 71, no. PART 05, 1 May 1990 (1990-05-01), pages 1243 - 1246, XP000406161
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