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Title:
EFFECTOR TYPE REGULATORY T CELL DETECTING METHOD, EFFECTOR TYPE REGULATORY T CELL ANALYZING DEVICE, EFFECTOR TYPE REGULATORY T CELL ANALYZING SYSTEM, AND PROGRAM
Document Type and Number:
WIPO Patent Application WO/2018/190340
Kind Code:
A1
Abstract:
In this method for detecting effector type regulatory T cells using flow cytometry: a control sample containing a population of CD4-positive T cells is measured; on the basis of the control sample measurement results, a FOXP3 quantity serving as a dividing reference when a population of FOXP3-positive T cells contained in the population of CD4-positive T cells is divided in accordance with a prescribed ratio is acquired as a reference value to be used when measuring a biological sample; a biological sample containing a population of CD4-positive T cells is measured; and on the basis of the biological sample measurement results, effector type regulatory T cells are detected from a population of cells in which the FOXP3 quantity is greater than the reference value.

Inventors:
NISHIKAWA, Hiroyoshi (Exploratory Oncology Research & Clinical Trial Center NATIONAL CANCER CENTER JAPAN, 5-1, Kashiwanoha 6-chome, Kashiwa-sh, Chiba 77, 〒2778577, JP)
KOBAYASHI, Tamiyo (5-1 Wakinohama-Kaigandori 1-chome, Chuo-ku, Kobe-sh, Hyogo 73, 〒6510073, JP)
Application Number:
JP2018/015094
Publication Date:
October 18, 2018
Filing Date:
April 10, 2018
Export Citation:
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Assignee:
NATIONAL CANCER CENTER JAPAN (1-1 Tsukiji 5-chome, Chuo-ku Tokyo, 45, 〒1040045, JP)
SYSMEX CORPORATION (5-1 Wakinohama-Kaigandori 1-chome, Chuo-ku Kobe-sh, Hyogo 73, 〒6510073, JP)
International Classes:
G01N33/49; C12M1/34; C12Q1/04; G01N33/483
Domestic Patent References:
WO2016010114A12016-01-21
Foreign References:
JP2016532865A2016-10-20
Other References:
NISHIKAWA, HAKUYOSHI: "Criteria of selective adaptation of cancer immunotherapy on the basis of novel immunological classification of colorectal cancer", RESEARCH PERFORMANCE REPORT ON SCIENTIFIC RESEARCH FUNDS RAISING BUSINESS, 10 June 2016 (2016-06-10), pages 1 - 6, XP009516771
HIRAHARA, K. ET AL.: "The Majority of Human Peripheral Blood CD 4+ CD 25hlgh Foxp3+ Regulatory T Cells Bear Functional Skin-Homing Receptors", JOURNAL OF IMMUNOLOGY, vol. 177, 2006, pages 4488 - 4494, XP055233375, DOI: 10.4049/jimmunol.177.7.4488
WARD, S. T. ET AL.: "A method for conducting suppression assays using small numbers of tissue-isolated regulatory T cells", METHODSX, vol. 1, 1 January 2014 (2014-01-01), pages 168 - 174, XP055632527, ISSN: 2215-0161, DOI: 10.1016/j.mex.2014.08.012
HIROYOSHI NISHIKAWA: "RegulatoryT cells in cancer immunotherapy", CURRENT OPINION IN IMMUNOLOGY, vol. 27, 2014, pages 1 - 7
Attorney, Agent or Firm:
SHIBANO, Masanori (Shibano Patent Office, 9F Mitsui Seimei Kobe Sannomiya Building 119, Ito-machi, Chuo-ku, Kobe-sh, Hyogo 32, 〒6500032, JP)
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