Title:
EFFICIENT GENETIC ENGINEERING VECTOR
Document Type and Number:
WIPO Patent Application WO/2019/149288
Kind Code:
A1
Abstract:
Provided is a nucleotide construct with 5' to 3' as represented by T-F-GOI; T-GOI-F; F-T-GOI; F-GOI-T; GOI-T-F; GOI-F-T; F-GOI; GOI-F; or P-F-GOI; P-GOI-F; F-P-GOI; F-GOI-P; GOI-P-F; GOI-F-P; F-GOI; and GOI-F respectively. T is an exogenous terminator; and F is a gene fragment represented by B-C-Marker-B, wherein B is a site-specific recombination site; C is a recombinant enzyme expression cassette corresponding to the site-specific recombination site; Marker is a marker gene expression cassette; GOI is a foreign gene expression cassette; and P is an exogenous promoter. A large number of foreign genes can be integrated into the genome using the nucleotide construct.
Inventors:
JIANG YOUWEI (CN)
LI YUNFEI (CN)
LI YUNFEI (CN)
Application Number:
PCT/CN2019/074640
Publication Date:
August 08, 2019
Filing Date:
February 02, 2019
Export Citation:
Assignee:
HANGZHOU GENEKINE BIOTECH CO LTD (CN)
International Classes:
C12N15/90; C12N1/16
Foreign References:
| CN104981542A | 2015-10-14 | |||
| CN101855356A | 2010-10-06 | |||
| CN106191041A | 2016-12-07 | |||
| CN104603273A | 2015-05-06 | |||
| CN104204205A | 2014-12-10 | |||
| CN102051379A | 2011-05-11 | |||
| CN101974547A | 2011-02-16 | |||
| CN106978416A | 2017-07-25 |
Attorney, Agent or Firm:
XU & PARTNERS, LLC. (CN)
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