Title:
EFFICIENT INDUCTION OF PLURIPOTENT STEM CELLS USING SMALL MOLECULE COMPOUNDS
Document Type and Number:
WIPO Patent Application WO/2010/033906
Kind Code:
A3
Abstract:
The disclosure features a method of producing a reprogrammed cell (e.g. an induced pluripotent stem cell or an undifferentiated cell) from a differentiated (e.g. somatic) cell. In some embodiments, the methods includes contacting a differentiated (e.g. somatic cell) with a TGFBR1 inhibitor or anti-TGF-β-antibody to produce a reprogrammed cell (e.g. pluripotent stem cell or undifferentiated cell). Embodiments of the present invention relate to a reprogrammed cell and methods and compositions for producing a chemically produced reprogrammed cell or populations thereof.
Inventors:
ICHIDA JUSTIN (US)
BLANCHARD JOEL (US)
LAM KELVIN (US)
RUBIN LEE (US)
EGGAN KEVIN (US)
BLANCHARD JOEL (US)
LAM KELVIN (US)
RUBIN LEE (US)
EGGAN KEVIN (US)
Application Number:
PCT/US2009/057669
Publication Date:
August 12, 2010
Filing Date:
September 21, 2009
Export Citation:
Assignee:
HARVARD COLLEGE (US)
ICHIDA JUSTIN (US)
BLANCHARD JOEL (US)
LAM KELVIN (US)
RUBIN LEE (US)
EGGAN KEVIN (US)
ICHIDA JUSTIN (US)
BLANCHARD JOEL (US)
LAM KELVIN (US)
RUBIN LEE (US)
EGGAN KEVIN (US)
International Classes:
C12N5/074
Domestic Patent References:
| WO2009102983A2 | 2009-08-20 |
Other References:
ICHIDA JUSTIN K ET AL: "A Small-Molecule Inhibitor of Tgf-beta Signaling Replaces Sox2 in Reprogramming by Inducing Nanog", CELL STEM CELL, vol. 5, no. 5, November 2009 (2009-11-01), pages 491 - 503, XP002564351, ISSN: 1934-5909
MAHERALI NIMET ET AL: "Tgf beta Signal Inhibition Cooperates in the Induction of iPSCs and Replaces Sox2 and cMyc", CURRENT BIOLOGY, vol. 19, no. 20, November 2009 (2009-11-01), pages 1718 - 1723, XP002564352, ISSN: 0960-9822
HUANGFU DANWEI ET AL: "Induction of pluripotent stem cells from primary human fibroblasts with only Oct4 and Sox2", NATURE BIOTECHNOLOGY, NATURE PUBLISHING GROUP, NEW YORK, NY, US, vol. 26, no. 11, 1 November 2008 (2008-11-01), pages 1269 - 1275, XP002534948, ISSN: 1087-0156, [retrieved on 20081012]
SHI YAN ET AL: "A combined chemical and genetic approach for the generation of induced pluripotent stem cells", CELL STEM CELL, vol. 2, no. 6, June 2008 (2008-06-01), pages 525 - 528, XP002564353, ISSN: 1934-5909
MIKKELSEN TARJEI S ET AL: "Dissecting direct reprogramming through integrative genomic analysis", NATURE (LONDON), vol. 454, no. 7200, July 2008 (2008-07-01), pages 49, XP002564354, ISSN: 0028-0836
HUANGFU DANWEI ET AL: "Induction of pluripotent stem cells by defined factors is greatly improved by small-molecule compounds", NATURE BIOTECHNOLOGY, NATURE PUBLISHING GROUP, NEW YORK, NY, US, vol. 26, no. 7, 1 July 2008 (2008-07-01), pages 795 - 797, XP002502536, ISSN: 1087-0156, [retrieved on 20080622]
MAHERALI NIMET ET AL: "Tgf beta Signal Inhibition Cooperates in the Induction of iPSCs and Replaces Sox2 and cMyc", CURRENT BIOLOGY, vol. 19, no. 20, November 2009 (2009-11-01), pages 1718 - 1723, XP002564352, ISSN: 0960-9822
HUANGFU DANWEI ET AL: "Induction of pluripotent stem cells from primary human fibroblasts with only Oct4 and Sox2", NATURE BIOTECHNOLOGY, NATURE PUBLISHING GROUP, NEW YORK, NY, US, vol. 26, no. 11, 1 November 2008 (2008-11-01), pages 1269 - 1275, XP002534948, ISSN: 1087-0156, [retrieved on 20081012]
SHI YAN ET AL: "A combined chemical and genetic approach for the generation of induced pluripotent stem cells", CELL STEM CELL, vol. 2, no. 6, June 2008 (2008-06-01), pages 525 - 528, XP002564353, ISSN: 1934-5909
MIKKELSEN TARJEI S ET AL: "Dissecting direct reprogramming through integrative genomic analysis", NATURE (LONDON), vol. 454, no. 7200, July 2008 (2008-07-01), pages 49, XP002564354, ISSN: 0028-0836
HUANGFU DANWEI ET AL: "Induction of pluripotent stem cells by defined factors is greatly improved by small-molecule compounds", NATURE BIOTECHNOLOGY, NATURE PUBLISHING GROUP, NEW YORK, NY, US, vol. 26, no. 7, 1 July 2008 (2008-07-01), pages 795 - 797, XP002502536, ISSN: 1087-0156, [retrieved on 20080622]
Attorney, Agent or Firm:
RESNICK, David et al. (100 Summer StreetBoston, Massachusetts, US)
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