Title:
EFFICIENT METHOD OF DERIVING ENDOTHELIAL CELLS
Document Type and Number:
WIPO Patent Application WO/2014/192925
Kind Code:
A1
Abstract:
Provided is a method for deriving endothelial cells from pluripotent stem cells, including the following steps: a) a step in which differential stimulus is imparted to pluripotent stem cells to form cell clusters containing at least 20% endothelial precursor cells from pluripotent stem cell; b) a step in which said cell cluster is cultured in a medium containing cAMP and VEGF, to a state in which the proportion of all cells which are endothelial precursor cells is at least 40%; and c) a step in which the cell clusters obtained in b) are cultivated in a medium containing VEGF to derive endothelial cells from endothelial precursor cells.
Inventors:
YAMASHITA JUN (JP)
IKUNO TAKESHI (JP)
MATSUNAGA TAICHI (JP)
IKUNO TAKESHI (JP)
MATSUNAGA TAICHI (JP)
Application Number:
PCT/JP2014/064442
Publication Date:
December 04, 2014
Filing Date:
May 30, 2014
Export Citation:
Assignee:
IHEART JAPAN CORP (JP)
International Classes:
C12N5/077
Domestic Patent References:
| WO2009120762A2 | 2009-10-01 | |||
| WO2013012087A1 | 2013-01-24 |
Foreign References:
| JP2005211040A | 2005-08-11 |
Other References:
T.YURUGI-KOBAYASHI ET AL., ARTERIOSCLER THROMB VASC BIOL., vol. 26, 2006, pages 1977 - 1984
TAICHI MATSUNAGA ET AL., REGENERATIVE MEDICINE, vol. 10, 2011, pages 181
TAICHI MATSUNAGA ET AL., REGENERATIVE MEDICINE, vol. 10, 2011, pages 181
Attorney, Agent or Firm:
KAWAGUCHI, Yoshiyuki et al. (JP)
Yoshiyuki Kawaguchi (JP)
Yoshiyuki Kawaguchi (JP)
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