Title:
ELECTROCHEMICAL DETECTION METHOD BASED ON SCREEN-PRINTED ELECTRODE
Document Type and Number:
WIPO Patent Application WO/2023/020434
Kind Code:
A1
Abstract:
An electrochemical detection method based on a screen-printed electrode. The method specifically comprises the following steps: S1, the preparation of a protein binding group, involving: treating a working electrode on a screen-printed electrode substrate, wherein the protein binding group is adhered to the working electrode; S2, basic protein reaction, involving: adding a basic protein, and an active group in the basic protein reacting with the protein binding group in step S1; S3, basic protein blocking, involving: performing, by using a blocking solution, a blocking treatment on the basic protein which has been subjected to reaction in step S2, so as to obtain a screen-printed electrode to be subjected to detection; and S4, electrochemical detection, involving: adding a detection reagent that contains a target protein, performing a specific reaction on the basic protein and the target protein, and performing electrochemical analysis on data collected from the working electrode after reaction, so as to obtain a detection result. Data is collected for electrochemical analysis, such that results of different detection items can be obtained according to different basic proteins and target proteins; and the electrochemical detection method has high sensitivity.
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Inventors:
XU XINGSHANG (CN)
CHEN JEFFERY (CN)
WANG LONG (CN)
CHEN JEFFERY (CN)
WANG LONG (CN)
Application Number:
PCT/CN2022/112506
Publication Date:
February 23, 2023
Filing Date:
August 15, 2022
Export Citation:
Assignee:
LANSION BIOTECHNOLOGY CO LTD (CN)
International Classes:
G01N27/26
Foreign References:
CN107677719A | 2018-02-09 | |||
CN111936854A | 2020-11-13 | |||
CN109470756A | 2019-03-15 | |||
CN112394174A | 2021-02-23 | |||
CN107144617A | 2017-09-08 | |||
JP2008101949A | 2008-05-01 | |||
CN112611793A | 2021-04-06 | |||
US20160202250A1 | 2016-07-14 | |||
CN109490399A | 2019-03-19 |
Other References:
ZHENG, QIQI ET AL.: "An anti-passivation ink for the preparation of electrodes for use in electrochemical immunoassays", JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B (BIOMEDICINE & BIOTECHNOLOGY), vol. 19, no. 9, 30 September 2018 (2018-09-30), pages 726 - 734, XP036589119, ISSN: 1862-1783, DOI: 10.1631/jzus.B1700510
Attorney, Agent or Firm:
NANJING ZHENGLIAN INTELLECTUAL PROPERTY AGENCY CO., LTD. (CN)
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