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Title:
ELECTRON MEDIATOR MODIFYING ENZYME, AND ENZYME ELECTRODE, SPECTROSCOPIC ANALYSIS KIT, AND ENZYME TEST PAPER USING SAME
Document Type and Number:
WIPO Patent Application WO/2018/062542
Kind Code:
A1
Abstract:
Disclosed is an electron mediator modifying enzyme represented by structural formula (I), said electron mediator modifying enzyme having a redox enzyme E, a phenazine derivative, and a linker region L in which the redox enzyme E and the phenazine derivative are coupled. In addition, in structural formula (I), Y represents a linear or branched alkyl group optionally having a C1-5 substituent, and R1 to R8 each independently represent a hydrogen atom, an alkyl group optionally having a substituent, or an alkoxy group, a hydroxyl group, a halogen atom, a nitro group, or an amino group optionally having a substituent, at least one of which is the linker region L. In addition, disclosed are an enzyme electrode, a spectroscopic analysis kit, and an enzyme test paper in which the electron mediator modifying enzyme is used.

Inventors:
SHIMAZAKI Junko (1-13-16 Minami, Meguro-k, Tokyo 13, 〒1520013, JP)
OGATA Hideki (2025-5 Tabaru, Mashiki-machi, Kamimashiki-gu, Kumamoto 02, 〒8612202, JP)
WATANABE Eiji (2025-5 Tabaru, Mashiki-machi, Kamimashiki-gu, Kumamoto 02, 〒8612202, JP)
Application Number:
JP2017/035676
Publication Date:
April 05, 2018
Filing Date:
September 29, 2017
Export Citation:
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Assignee:
ULTIZYME INTERNATIONAL LTD. (1-13-16, Minami Meguro-k, Tokyo 13, 〒1520013, JP)
DOJINDO LABORATORIES (2025-5, Tabaru Mashiki-machi, Kamimashiki-gu, Kumamoto 02, 〒8612202, JP)
International Classes:
C12N9/02; C12M1/34; C12N9/04; C12Q1/26; C12Q1/32; C12Q1/54; C12Q1/60; G01N21/78; G01N27/327; G01N27/416; G01N31/00; G01N31/22
Foreign References:
JP2005073630A2005-03-24
JP2011515686A2011-05-19
Other References:
YOMO, TETSUYA ET AL.: "Preparation and kinetic properties of 5-ethylphenazine-glucose-dehydrogenase-NAD+ conjugate, a semisynthetic glucose oxidase", EUROPEAN JOURNAL OF BIOCHMEMISTRY, vol. 200, 1991, pages 759 - 766, XP055480382
YABUKI, SOICHI: "Application of chemically modified enzymes to biosensors", MATERIALS INTEGRATION, vol. 21, no. 5, 2008, pages 191 - 196
Attorney, Agent or Firm:
NAKASHIMA Kazuaki (Room 202, States-Tenjin-Minami 11-9, Kiyokawa 1-chome, Chuo-ku, Fukuoka-sh, Fukuoka 05, 〒8100005, JP)
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