Title:
ELECTROPHORETIC ANALYSIS METHOD, ELECTROPHORETIC ANALYSIS DEVICE, AND ELECTROPHORETIC ANALYSIS PROGRAM
Document Type and Number:
WIPO Patent Application WO/2018/163610
Kind Code:
A1
Abstract:
In the present invention, RNA quality is evaluated on the basis of a feature value corresponding to the position of a degradation product peak P3 that appears in conjunction with degradation of RNA in a region on a low-molecular-weight side relative to an 18S peak P1 in an electrophoresis waveform. The degradation product peak P3 appears in conjunction with degradation of RNA in a region on the low-molecular-weight side relative to the 18S peak P1 after RNA has degraded to a degree that the 18S peak P1 disappears, and the degradation product peak P3 shifts to the low-molecular-weight side as the RNA degrades. The quality even of degraded RNA can therefore be evaluated with good precision on the basis of a feature value that corresponds to the position of the degradation product peak P3.
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Inventors:
CHINOMI KENTA (JP)
Application Number:
PCT/JP2018/001395
Publication Date:
September 13, 2018
Filing Date:
January 18, 2018
Export Citation:
Assignee:
SHIMADZU CORP (JP)
International Classes:
G01N27/447; C12Q1/68; G01N33/50
Foreign References:
| JP2012501458A | 2012-01-19 | |||
| JP2007526979A | 2007-09-20 | |||
| JP2015519887A | 2015-07-16 | |||
| JP4664280B2 | 2011-04-06 | |||
| JPS5620382B2 | 1981-05-13 |
Other References:
FLEIGE, SIMONE ET AL.: "RNA integrity and the effect on the real-time qRT-PCR performance", MOLECULAR ASPECTS OF MEDICINE, vol. 27, no. 2-3, April 2006 (2006-04-01), pages 126 - 139, XP055542654
MUELLER, ODILO ET AL.: "RNA Integrity Number (RIN) - Standardization of RNA Quality Control", AGILENT TECHNOLOGIES, 1 May 2004 (2004-05-01), pages 1 - 8, XP055542656, Retrieved from the Internet [retrieved on 20180315]
FONTANESI, L. ET AL.: "Evaluation of post mortem stability of porcine skeletal muscle RNA", MEAT SCIENCE, vol. 80, no. 4, 14 June 2008 (2008-06-14), pages 1345 - 1351, XP025465925
ANDREAS SCHROEDER: "The RIN: an RNA integrity number for assigning integrity values to RNA measurements", BIOMED CENTRAL, 31 January 2006 (2006-01-31), Retrieved from the Internet
VLADIMIR DENISOV4 OTHERS: "Development and validation of RQI: an RNA quality indicator for the Experion automated electrophoresis system", 9 February 2017, CALIPER LIFE SCIENCES, INC.
See also references of EP 3594674A4
MUELLER, ODILO ET AL.: "RNA Integrity Number (RIN) - Standardization of RNA Quality Control", AGILENT TECHNOLOGIES, 1 May 2004 (2004-05-01), pages 1 - 8, XP055542656, Retrieved from the Internet
FONTANESI, L. ET AL.: "Evaluation of post mortem stability of porcine skeletal muscle RNA", MEAT SCIENCE, vol. 80, no. 4, 14 June 2008 (2008-06-14), pages 1345 - 1351, XP025465925
ANDREAS SCHROEDER: "The RIN: an RNA integrity number for assigning integrity values to RNA measurements", BIOMED CENTRAL, 31 January 2006 (2006-01-31), Retrieved from the Internet
VLADIMIR DENISOV4 OTHERS: "Development and validation of RQI: an RNA quality indicator for the Experion automated electrophoresis system", 9 February 2017, CALIPER LIFE SCIENCES, INC.
See also references of EP 3594674A4
Attorney, Agent or Firm:
YOSHIMOTO, Tsutomu et al. (JP)
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