Title:
ELECTROPORATION DEVICE AND METHOD FOR PRODUCING CELLS WITH INTRODUCED FOREIGN SUBSTANCE
Document Type and Number:
WIPO Patent Application WO/2020/130106
Kind Code:
A1
Abstract:
Provided are: an electroporation device which, compared to devices in which electroporation is performed by reciprocating a solution (liquid droplets) containing a foreign substance and cells in an oil, facilitates an operation for collecting a sample after a reaction; and a method for producing cells with an introduced foreign substance. The electroporation device (1) is provided with a retaining unit (2), an electric discharge generation unit (3), an electrically conductive unit (4), and an electric energy control unit (5). The retaining unit (2) retains a solution containing a foreign substance and cells. The electric discharge generation unit (3) has a pair of electrodes disposed at a predetermined interval and generates an arc discharge between the pair of electrodes. The electrically conductive unit (4) electrically connects the retaining unit (2) and the electric discharge generation unit (3), and supplies a pulse current generated by the electric discharge generation unit (3) to the retaining unit (2). The electric energy control unit (5) controls the electric energy of the pulse current supplied to the retaining unit (2).
Inventors:
NUMANO RIKA (JP)
SHINOZAKI RYUTO (JP)
SHINOZAKI RYUTO (JP)
Application Number:
PCT/JP2019/049952
Publication Date:
June 25, 2020
Filing Date:
December 19, 2019
Export Citation:
Assignee:
NATIONAL UNIV CORPORATION TOYOHASHI UNIV OF TECHNOLOGY (JP)
International Classes:
C12M1/00; C12M1/42; C12N5/10; C12N13/00; C12N15/87
Domestic Patent References:
| WO2014092164A1 | 2014-06-19 |
Foreign References:
| JP2017104073A | 2017-06-15 | |||
| JP2001187384A | 2001-07-10 | |||
| US20160159673A1 | 2016-06-09 |
Other References:
REBERSEK, M. ET AL.: "Generator and setup for emulating exposures of biological samples to lightning strokes", IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING, vol. 62, no. 10, 2015, pages 2535 - 2543, XP011668919, ISSN: 0018-9294, DOI: 10.1109/TBME.2015.2437359
Attorney, Agent or Firm:
YAMAMOTO, Hisashi et al. (JP)
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