Title:
ENDORIBONUCLEASE, PROTEIN, POLYNUCLEOTIDE, EXPRESSION VECTOR, TRANSFORMANT, COMPLEX, RNA FRAGMENT PRODUCTION METHOD, RNA CLEAVAGE METHOD, AND CELL CONTROL METHOD
Document Type and Number:
WIPO Patent Application WO/2023/248870
Kind Code:
A1
Abstract:
This endoribonuclease comprises the amino acid sequence of (a) or (b) below and hydrolyzes the phosphodiester bond between U and A in 5'-UACU-3' or 5'-UACG-3' included in RNA. (a) An amino acid sequence represented by SEQ ID NO: 1. (b) An amino acid sequence having 90% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1.
More Like This:
Inventors:
NODA NAOHIRO (JP)
YOKOTA AKIKO (JP)
OKABE TAKUMA (JP)
AOI RIE (JP)
ISHITSUKA HIROKO (JP)
JIANG YUNONG (JP)
TSUNEDA SATOSHI (JP)
YOKOTA AKIKO (JP)
OKABE TAKUMA (JP)
AOI RIE (JP)
ISHITSUKA HIROKO (JP)
JIANG YUNONG (JP)
TSUNEDA SATOSHI (JP)
Application Number:
PCT/JP2023/021892
Publication Date:
December 28, 2023
Filing Date:
June 13, 2023
Export Citation:
Assignee:
AIST (JP)
UNIV WASEDA (JP)
UNIV WASEDA (JP)
International Classes:
C12N15/55; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/16; C12N15/63; C12P19/34
Foreign References:
| JP2018191565A | 2018-12-06 |
Other References:
DATABASE UniProtKB 29 September 2021 (2021-09-29), ANONYMOUS : "RecName: Full=mRNA interferase {ECO:0000256|PIRNR:PIRNR033490}", XP093119990, Database accession no. A0A5W0NR27
MIYAMOTO TATSUKI, KATO YUKA, SEKIGUCHI YUJI, TSUNEDA SATOSHI, NODA NAOHIRO: "Characterization of MazF-Mediated Sequence-Specific RNA Cleavage in Pseudomonas putida Using Massive Parallel Sequencing", PLOS ONE, PUBLIC LIBRARY OF SCIENCE, US, vol. 11, no. 2, 17 February 2016 (2016-02-17), US , pages e0149494, XP093119993, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0149494
LEE MIN WOO, ROGERS ELIZABETH E., STENGER DRAKE C.: "Xylella fastidiosa Plasmid-Encoded PemK Toxin Is an Endoribonuclease", PHYTOPATHOLOGY, AMERICAN PHYTOPATHOLOGICAL SOCIETY, US, vol. 102, no. 1, 1 January 2012 (2012-01-01), US , pages 32 - 40, XP093119996, ISSN: 0031-949X, DOI: 10.1094/PHYTO-05-11-0150
DATABASE UniProtKB 29 September 2021 (2021-09-29), ANONYMOUS : "RecName: Full=SpoVT-AbrB domain-containing protein {ECO:0000259|PROSITE:PS51740}", XP093120002, Database accession no. A9MQQ4
DATABASE UniProtKB 19 January 2022 (2022-01-19), ANONYMOUS : "SubName: Full=Antitoxin {ECO:0000313|EMBL:EBV9897159.1}", XP093120005, Database accession no. A0A5Z6PKN6
CHAN WAI TING, ESPINOSA MANUEL, YEO CHEW CHIENG: "Keeping the Wolves at Bay: Antitoxins of Prokaryotic Type II Toxin-Antitoxin Systems", FRONTIERS IN MOLECULAR BIOSCIENCES, vol. 3, XP093120006, ISSN: 2296-889X, DOI: 10.3389/fmolb.2016.00009
MIYAMOTO TATSUKI, KATO YUKA, SEKIGUCHI YUJI, TSUNEDA SATOSHI, NODA NAOHIRO: "Characterization of MazF-Mediated Sequence-Specific RNA Cleavage in Pseudomonas putida Using Massive Parallel Sequencing", PLOS ONE, PUBLIC LIBRARY OF SCIENCE, US, vol. 11, no. 2, 17 February 2016 (2016-02-17), US , pages e0149494, XP093119993, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0149494
LEE MIN WOO, ROGERS ELIZABETH E., STENGER DRAKE C.: "Xylella fastidiosa Plasmid-Encoded PemK Toxin Is an Endoribonuclease", PHYTOPATHOLOGY, AMERICAN PHYTOPATHOLOGICAL SOCIETY, US, vol. 102, no. 1, 1 January 2012 (2012-01-01), US , pages 32 - 40, XP093119996, ISSN: 0031-949X, DOI: 10.1094/PHYTO-05-11-0150
DATABASE UniProtKB 29 September 2021 (2021-09-29), ANONYMOUS : "RecName: Full=SpoVT-AbrB domain-containing protein {ECO:0000259|PROSITE:PS51740}", XP093120002, Database accession no. A9MQQ4
DATABASE UniProtKB 19 January 2022 (2022-01-19), ANONYMOUS : "SubName: Full=Antitoxin {ECO:0000313|EMBL:EBV9897159.1}", XP093120005, Database accession no. A0A5Z6PKN6
CHAN WAI TING, ESPINOSA MANUEL, YEO CHEW CHIENG: "Keeping the Wolves at Bay: Antitoxins of Prokaryotic Type II Toxin-Antitoxin Systems", FRONTIERS IN MOLECULAR BIOSCIENCES, vol. 3, XP093120006, ISSN: 2296-889X, DOI: 10.3389/fmolb.2016.00009
Attorney, Agent or Firm:
KIMURA Mitsuru et al. (JP)
Download PDF: