Title:
ENDOTOXIN DETECTION DEVICE AND ENDOTOXIN DETECTION METHOD
Document Type and Number:
WIPO Patent Application WO/2020/184482
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide: a device for detecting endotoxin within a short time and in a simple manner without the need to use an expensive reagent; and a method for detecting endotoxin. An endotoxin detection device according to the present invention is characterized by being provided with: an area section containing an electrolysis solution; a partitioning member which partitions the area section into two sections so that the two sections can communicate with each other through nanopores; a first electrode which is arranged in a first section; a second electrode which is arranged in a second section and is electrically connected to the first electrode; an electrolysis solution flow generation means for moving the electrolysis solution in the first section to the second section through the nanopores; an application means for applying a voltage between the first electrode and the second electrode; and a monitoring means for monitoring an electric current.
Inventors:
ITO TAKAHIRO (JP)
INOUE KUMI (JP)
ITO KENTARO (JP)
SHIKU HITOSHI (JP)
MATSUE TOMOKAZU (JP)
INOUE KUMI (JP)
ITO KENTARO (JP)
SHIKU HITOSHI (JP)
MATSUE TOMOKAZU (JP)
Application Number:
PCT/JP2020/009859
Publication Date:
September 17, 2020
Filing Date:
March 06, 2020
Export Citation:
Assignee:
UNIV TOHOKU (JP)
International Classes:
G01N15/12; G01N27/00
Foreign References:
| JP2018072181A | 2018-05-10 | |||
| JP2015517671A | 2015-06-22 | |||
| US2656508A | 1953-10-20 | |||
| JPS5480411A | 1979-06-27 | |||
| JP2008536124A | 2008-09-04 | |||
| US20130092541A1 | 2013-04-18 | |||
| JPS5942451A | 1984-03-09 | |||
| JP2018072331A | 2018-05-10 | |||
| JP2017037043A | 2017-02-16 |
Other References:
SANTOS, NUNO C. AND SILVA ANA C, CASTANHO MIGUEL A R B, MARTINS-SILVA ] J: "Evaluation of Lipopolysaccharide Aggregation by Light Scattering Spectroscopy", CHEMBIOCHEM, vol. 4, 3 January 2003 (2003-01-03), pages 96 - 100, XP055739397
ITO, TAKASHI ET AL.: "Simultaneous Determination of the Size and Surface Charge of Individual Nanoparticles Using a Carbon Nanotube-Based Coulter Counter", ANALYTICAL CHEMISTRY, vol. 75, no. 10, 15 May 2003 (2003-05-15), pages 2399 - 2406, XP001170911, DOI: 10.1021/ac034072v
ITO, KENTARO ET AL.: "A Nanopore-based Endotoxin Sensor", THE JAPAN SOC. FOR ANALYTICAL CHEMISTRY, vol. 68, no. 8, 5 August 2019 (2019-08-05), pages 575 - 580, XP055739404
See also references of EP 3835755A4
ITO, TAKASHI ET AL.: "Simultaneous Determination of the Size and Surface Charge of Individual Nanoparticles Using a Carbon Nanotube-Based Coulter Counter", ANALYTICAL CHEMISTRY, vol. 75, no. 10, 15 May 2003 (2003-05-15), pages 2399 - 2406, XP001170911, DOI: 10.1021/ac034072v
ITO, KENTARO ET AL.: "A Nanopore-based Endotoxin Sensor", THE JAPAN SOC. FOR ANALYTICAL CHEMISTRY, vol. 68, no. 8, 5 August 2019 (2019-08-05), pages 575 - 580, XP055739404
See also references of EP 3835755A4
Attorney, Agent or Firm:
SUGIMURA Kenji (JP)
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