Title:
ENGINEERED ALDOLASE POLYPEPTIDES AND USES THEREOF
Document Type and Number:
WIPO Patent Application WO/2018/219108
Kind Code:
A1
Abstract:
Provided herein are engineered polypeptides that are useful for the asymmetric synthesis of β-hydroxy-α-amino acids under industrial-relevant conditions. Also provided are polynucleotides encoding engineered polypeptides, host cells capable of expressing engineered polypeptides, and methods of producing β-hydroxy-α-amino acids using engineered polypeptides. Compared to other processes of preparation, the use of the engineered polypeptides for the preparation of β-hydroxy-α-amino acids results in high purity of the desired stereoisomers, mild reaction conditions, low pollution and low energy consumption.
Inventors:
CHEN HAIBIN (CN)
BONG YONG KOY (CN)
CAI BAOQIN (CN)
XU QING (CN)
SHEN TIANRAN (CN)
ZHOU AMENG (CN)
YANG JIADONG (CN)
YANG ZHUHONG (CN)
JI YAOYAO (CN)
ZHANG YINGXIN (CN)
BONG YONG KOY (CN)
CAI BAOQIN (CN)
XU QING (CN)
SHEN TIANRAN (CN)
ZHOU AMENG (CN)
YANG JIADONG (CN)
YANG ZHUHONG (CN)
JI YAOYAO (CN)
ZHANG YINGXIN (CN)
Application Number:
PCT/CN2018/086228
Publication Date:
December 06, 2018
Filing Date:
May 09, 2018
Export Citation:
Assignee:
ENZYMASTER NINGBO BIO ENG CO LTD (CN)
International Classes:
C07K14/00; C07K17/00; C12P7/40
Other References:
DATABASE NCBI GenBank [O] 15 May 2017 (2017-05-15), "low specificity L-threonine aldolase [Pseudomonas fluorescens", XP055556910, Database accession no. WP_057714849.1
DATABASE NCBI [O] 10 August 2005 (2005-08-10), MISONO, H. ET AL.: "phenylserine aldolase", XP055556916, Database accession no. BAD91544.1
KIMURA, T. ET AL.: "Enzymatic Synthesis of beta-Hydroxy-alpha-amino Acids Based on Recombinant D- and L-Threonine Aldolases", J. AM. CHEM. SOC., vol. 119, no. 49, 31 December 1997 (1997-12-31), pages 11734 - 11742, XP055556922
LIU, J. Q. ET AL.: "Diversity of microbial threonine aldolases and their application", JOURNAL OF MOLECULAR CATALYSIS B: ENZYMATIC., vol. 10, no. 1-3, 31 December 2000 (2000-12-31), pages 107 - 115, XP002451052
DUCKERS, N. ET AL.: "Threonine aldolases-screening, properties and applications in the synthesis of non-proteinogenic beta-hydroxy-alpha-amino acids", APPL. MICROBIOL. BIOTECHNOL., vol. 88, no. 2, 4 August 2010 (2010-08-04), pages 409 - 424, XP019841749
HAN, Y. Y. ET AL.: "Synthesis of p-Methylsulfonylphenylserine Copper", CHEMICAL INDUSTRY AND ENGINEERING., vol. 28, no. 2, 31 March 2011 (2011-03-31), pages 29 - 34, XP055627681
BROWN ET AL., J. BACTERIOL., vol. 194, 2012, pages 5991 - 5993
KIMURA, T, J. AM. CHEM. SOC., vol. 119, no. 49, 1997, pages 11734 - 11742
LIU, J. Q., JOURNAL OF MOLECULAR CATALYSIS B: ENZYMATIC., vol. 10, no. 1-3, 2000, pages 107 - 116
BULUT D ET AL., APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 99, no. 14, 2015, pages 5875 - 5883
KATERYNA F ET AL., APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 100, no. 6, 2016, pages 2579 - 2590
DUCKERS, N ET AL., APPL. MICROBIOL. BIOTECHNOL., vol. 88, no. 2, 2010, pages 409 - 424
STEINREIBER ET AL., TETRAHEDRON, vol. 63, no. 4, 2006, pages 918 - 926
NUCLEIC ACID RESEARCH, vol. 22, no. 22, 1994, pages 4673 - 4680
NEEDLEMANWUNSCH, J. MOL. BIOL., vol. 48, 1970, pages 443
PEARSONLIPMAN, PROC. NATL. ACAD. SCI. USA, vol. 85, 1988, pages 2444
"Current Protocols in Molecular Biology", 1998, GREENE PUBLISHING ASSOCIATES, INC. AND JOHN WILEY & SONS, INC., article "Current Protocols, a Joint Venture"
ALTSCHUL ET AL., J. MOL. BIOL., vol. 215, 1990, pages 403 - 410
ALTSCHUL ET AL., NUCLEIC ACIDS RES, 1977, pages 3389 - 3402
HENIKOFFHENIKOFF, PROC NATL ACAD SCI USA, vol. 89, 1989, pages 10915
SAMBROOK ET AL.: "Molecular Cloning: A Laboratory Manual", 2001, COLD SPRING HARBOR LABORATORY PRESS
"Biocatalysis for the Pharmaceutical Industry: Discovery, Development, and Manufacturing", 2009, JOHN WILEY & SONS ASIA (PTE) LTD.
BEAUCAGE ET AL., TET LETT, vol. 22, 1981, pages 1859 - 69
MATTHES ET AL.: "People", EMBO J, vol. 3, 1984, pages 801 - 05
DATABASE NCBI [O] 10 August 2005 (2005-08-10), MISONO, H. ET AL.: "phenylserine aldolase", XP055556916, Database accession no. BAD91544.1
KIMURA, T. ET AL.: "Enzymatic Synthesis of beta-Hydroxy-alpha-amino Acids Based on Recombinant D- and L-Threonine Aldolases", J. AM. CHEM. SOC., vol. 119, no. 49, 31 December 1997 (1997-12-31), pages 11734 - 11742, XP055556922
LIU, J. Q. ET AL.: "Diversity of microbial threonine aldolases and their application", JOURNAL OF MOLECULAR CATALYSIS B: ENZYMATIC., vol. 10, no. 1-3, 31 December 2000 (2000-12-31), pages 107 - 115, XP002451052
DUCKERS, N. ET AL.: "Threonine aldolases-screening, properties and applications in the synthesis of non-proteinogenic beta-hydroxy-alpha-amino acids", APPL. MICROBIOL. BIOTECHNOL., vol. 88, no. 2, 4 August 2010 (2010-08-04), pages 409 - 424, XP019841749
HAN, Y. Y. ET AL.: "Synthesis of p-Methylsulfonylphenylserine Copper", CHEMICAL INDUSTRY AND ENGINEERING., vol. 28, no. 2, 31 March 2011 (2011-03-31), pages 29 - 34, XP055627681
BROWN ET AL., J. BACTERIOL., vol. 194, 2012, pages 5991 - 5993
KIMURA, T, J. AM. CHEM. SOC., vol. 119, no. 49, 1997, pages 11734 - 11742
LIU, J. Q., JOURNAL OF MOLECULAR CATALYSIS B: ENZYMATIC., vol. 10, no. 1-3, 2000, pages 107 - 116
BULUT D ET AL., APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 99, no. 14, 2015, pages 5875 - 5883
KATERYNA F ET AL., APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 100, no. 6, 2016, pages 2579 - 2590
DUCKERS, N ET AL., APPL. MICROBIOL. BIOTECHNOL., vol. 88, no. 2, 2010, pages 409 - 424
STEINREIBER ET AL., TETRAHEDRON, vol. 63, no. 4, 2006, pages 918 - 926
NUCLEIC ACID RESEARCH, vol. 22, no. 22, 1994, pages 4673 - 4680
NEEDLEMANWUNSCH, J. MOL. BIOL., vol. 48, 1970, pages 443
PEARSONLIPMAN, PROC. NATL. ACAD. SCI. USA, vol. 85, 1988, pages 2444
"Current Protocols in Molecular Biology", 1998, GREENE PUBLISHING ASSOCIATES, INC. AND JOHN WILEY & SONS, INC., article "Current Protocols, a Joint Venture"
ALTSCHUL ET AL., J. MOL. BIOL., vol. 215, 1990, pages 403 - 410
ALTSCHUL ET AL., NUCLEIC ACIDS RES, 1977, pages 3389 - 3402
HENIKOFFHENIKOFF, PROC NATL ACAD SCI USA, vol. 89, 1989, pages 10915
SAMBROOK ET AL.: "Molecular Cloning: A Laboratory Manual", 2001, COLD SPRING HARBOR LABORATORY PRESS
"Biocatalysis for the Pharmaceutical Industry: Discovery, Development, and Manufacturing", 2009, JOHN WILEY & SONS ASIA (PTE) LTD.
BEAUCAGE ET AL., TET LETT, vol. 22, 1981, pages 1859 - 69
MATTHES ET AL.: "People", EMBO J, vol. 3, 1984, pages 801 - 05
Download PDF: