Title:
ENGINEERED CAS6 PROTEIN AND USES THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/029532
Kind Code:
A1
Abstract:
Provided are engineered Cas6 proteins with decreased ability to process DR sequences of guide RNAs and uses thereof.
Inventors:
YANG HUI (CN)
WANG XING (CN)
WANG ZIKANG (CN)
ZHANG RENXIA (CN)
YANG DONG (CN)
WANG XING (CN)
WANG ZIKANG (CN)
ZHANG RENXIA (CN)
YANG DONG (CN)
Application Number:
PCT/CN2022/089624
Publication Date:
March 09, 2023
Filing Date:
April 27, 2022
Export Citation:
Assignee:
HUIGENE THERAPEUTICS CO LTD (CN)
CENTER FOR EXCELLENCE IN BRAIN SCIENCE AND INTELLIGENCE TECH CHINESE ACADEMY OF SCIENCES (CN)
CENTER FOR EXCELLENCE IN BRAIN SCIENCE AND INTELLIGENCE TECH CHINESE ACADEMY OF SCIENCES (CN)
International Classes:
C12N15/90; C07K19/00; C12N15/62
Domestic Patent References:
| WO2019071274A1 | 2019-04-11 | |||
| WO2021076656A1 | 2021-04-22 |
Foreign References:
| CN110128546A | 2019-08-16 | |||
| CN110527697A | 2019-12-03 | |||
| CN108949831A | 2018-12-07 |
Other References:
JOHN R. SINNAMON, ET AL.: "Site-directed RNA repair of endogenous Mecp2 RNA in neurons", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, vol. 114, no. 44, 16 October 2017 (2017-10-16), pages E9395 - E9402, XP055588475, ISSN: 0027-8424, DOI: 10.1073/pnas.1715320114
RAUCH SIMONE, JONES KRYSTEN A., DICKINSON BRYAN C.: "Small Molecule-Inducible RNA-Targeting Systems for Temporal Control of RNA Regulation", ACS CENTRAL SCIENCE, vol. 6, no. 11, 25 November 2020 (2020-11-25), pages 1987 - 1996, XP055868003, ISSN: 2374-7943, DOI: 10.1021/acscentsci.0c00537
MARINA RYAN J., BRANNAN KRISTOPHER W., DONG KEVIN D., YEE BRIAN A., YEO GENE W.: "Evaluation of Engineered CRISPR-Cas-Mediated Systems for Site-Specific RNA Editing", CELL REPORTS, ELSEVIER INC, US, vol. 33, no. 5, 3 November 2020 (2020-11-03), US , pages 108350, XP055898294, ISSN: 2211-1247, DOI: 10.1016/j.celrep.2020.108350
SINNAMON JOHN R., KIM SUSAN Y., FISK JENNA R., SONG ZHEN, NAKAI HIROYUKI, JENG SOPHIA, MCWEENEY SHANNON K., MANDEL GAIL: "In Vivo Repair of a Protein Underlying a Neurological Disorder by Programmable RNA Editing", CELL REPORTS, ELSEVIER INC, US, vol. 32, no. 2, 1 July 2020 (2020-07-01), US , pages 107878, XP055962460, ISSN: 2211-1247, DOI: 10.1016/j.celrep.2020.107878
DANIEL CHAMMIRAN, WIDMARK ALBIN, RIGARDT DITTE, ÖHMAN MARIE: "Editing inducer elements increases A-to-I editing efficiency in the mammalian transcriptome", GENOME BIOLOGY, vol. 18, no. 1, 1 December 2017 (2017-12-01), XP055809499, DOI: 10.1186/s13059-017-1324-x
DAVID B. T. COX, JONATHAN S. GOOTENBERG, OMAR O. ABUDAYYEH, BRIAN FRANKLIN, MAX J. KELLNER, JULIA JOUNG, FENG ZHANG: "RNA editing with CRISPR-Cas13", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE, US, vol. 358, no. 6366, 24 November 2017 (2017-11-24), US , pages 1019 - 1027, XP055491658, ISSN: 0036-8075, DOI: 10.1126/science.aaq0180
RAUCH SIMONE, JONES KRYSTEN A., DICKINSON BRYAN C.: "Small Molecule-Inducible RNA-Targeting Systems for Temporal Control of RNA Regulation", ACS CENTRAL SCIENCE, vol. 6, no. 11, 25 November 2020 (2020-11-25), pages 1987 - 1996, XP055868003, ISSN: 2374-7943, DOI: 10.1021/acscentsci.0c00537
MARINA RYAN J., BRANNAN KRISTOPHER W., DONG KEVIN D., YEE BRIAN A., YEO GENE W.: "Evaluation of Engineered CRISPR-Cas-Mediated Systems for Site-Specific RNA Editing", CELL REPORTS, ELSEVIER INC, US, vol. 33, no. 5, 3 November 2020 (2020-11-03), US , pages 108350, XP055898294, ISSN: 2211-1247, DOI: 10.1016/j.celrep.2020.108350
SINNAMON JOHN R., KIM SUSAN Y., FISK JENNA R., SONG ZHEN, NAKAI HIROYUKI, JENG SOPHIA, MCWEENEY SHANNON K., MANDEL GAIL: "In Vivo Repair of a Protein Underlying a Neurological Disorder by Programmable RNA Editing", CELL REPORTS, ELSEVIER INC, US, vol. 32, no. 2, 1 July 2020 (2020-07-01), US , pages 107878, XP055962460, ISSN: 2211-1247, DOI: 10.1016/j.celrep.2020.107878
DANIEL CHAMMIRAN, WIDMARK ALBIN, RIGARDT DITTE, ÖHMAN MARIE: "Editing inducer elements increases A-to-I editing efficiency in the mammalian transcriptome", GENOME BIOLOGY, vol. 18, no. 1, 1 December 2017 (2017-12-01), XP055809499, DOI: 10.1186/s13059-017-1324-x
DAVID B. T. COX, JONATHAN S. GOOTENBERG, OMAR O. ABUDAYYEH, BRIAN FRANKLIN, MAX J. KELLNER, JULIA JOUNG, FENG ZHANG: "RNA editing with CRISPR-Cas13", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE, US, vol. 358, no. 6366, 24 November 2017 (2017-11-24), US , pages 1019 - 1027, XP055491658, ISSN: 0036-8075, DOI: 10.1126/science.aaq0180
Attorney, Agent or Firm:
SHANGHAI PATENT & TRADEMARK LAW OFFICE, LLC (CN)
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