Title:
ENGINEERED DECARBOXYLASE POLYPEPTIDES AND USES THEREOF IN PREPARING TYRAMINE AND DOPAMINE
Document Type and Number:
WIPO Patent Application WO/2019/192438
Kind Code:
A1
Abstract:
Provided herein are engineered decarboxylase polypeptides that are useful for catalyzing the decarboxylation of amino aicds such as L-tyrosine to produce tyramine or catalyzing the decarboxylation of L-DOPA to produce dopamine. Also provided is the preparation process of the engineered decarboxylase polypeptides as well as reaction process under industrial-relevant conditions. Also provided are polynucleotide sequences encoding the engineered decarboxylase polypeptides, and methods of producing tyramine or dopamine using the engineered decarboxylase polypeptides.
Inventors:
CHEN HAIBIN (CN)
BONG YONG KOY (CN)
XU QING (CN)
HU DONGXIAO (CN)
ZHANG CHENGXIAO (CN)
CAI BAOQIN (CN)
BONG YONG KOY (CN)
XU QING (CN)
HU DONGXIAO (CN)
ZHANG CHENGXIAO (CN)
CAI BAOQIN (CN)
Application Number:
PCT/CN2019/080901
Publication Date:
October 10, 2019
Filing Date:
April 01, 2019
Export Citation:
Assignee:
ENZYMASTER NINGBO BIO ENG CO LTD (CN)
International Classes:
C12N9/88; C12N1/21; C12N15/01; C12P13/00
Domestic Patent References:
| WO2012135389A2 | 2012-10-04 | |||
| WO2000047236A1 | 2000-08-17 |
Foreign References:
| CN103421734A | 2013-12-04 | |||
| EP1621626A1 | 2006-02-01 |
Other References:
SHUHEI N ET AL.: "Evaluation of Brachypodium distachyon L-Tyrosine Decarboxylase Using Tyrosine Over-Producing Saccharomyces cerevisiae", PLOS ONE, vol. 10, no. 5, 21 May 2015 (2015-05-21), pages e0125488, XP055510295, DOI: 10.1371/journal.pone.0125488
SHUHEI N ET AL.: "Evaluation of Brachypodium distachyon L-Tyrosine Decarboxylase Using L- Tyrosine Over-Producing Saccharomyces cerevisiae", PLOS ONE, vol. 10, no. 5, 21 May 2015 (2015-05-21), pages e0125488, XP055510295, DOI: 10.1371/journal.pone.0125488
See also references of EP 3775195A4
SHUHEI N ET AL.: "Evaluation of Brachypodium distachyon L-Tyrosine Decarboxylase Using L- Tyrosine Over-Producing Saccharomyces cerevisiae", PLOS ONE, vol. 10, no. 5, 21 May 2015 (2015-05-21), pages e0125488, XP055510295, DOI: 10.1371/journal.pone.0125488
See also references of EP 3775195A4
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