Title:
ENGINEERED ENZYMES WITH METHIONINE-GAMMA-LYASE ENZYMES AND PHARMACOLOGICAL PREPARATIONS THEROF
Document Type and Number:
WIPO Patent Application WO/2011/097381
Kind Code:
A3
Abstract:
Methods and composition related to the engineering of a novel protein with methionine-γ-lyase enzyme activity are described. For example, in certain aspects there may be disclosed a modified cystathionine-γ-lyase (CGL) comprising one or more amino acid substitutions and capable of degrading methionine. Furthermore, certain aspects of the invention provide compositions and methods for the treatment of cancer with methionine depletion using the disclosed proteins or nucleic acids.
Inventors:
GEORGIOU GEORGE (US)
STONE EVERETT (US)
STONE EVERETT (US)
Application Number:
PCT/US2011/023606
Publication Date:
October 25, 2018
Filing Date:
February 03, 2011
Export Citation:
Assignee:
UNIV TEXAS (US)
GEORGIOU GEORGE (US)
STONE EVERETT (US)
GEORGIOU GEORGE (US)
STONE EVERETT (US)
International Classes:
C12N9/88; A61K38/51; A61P35/00; C12N15/60
Foreign References:
| US20050036984A1 | 2005-02-17 |
Other References:
ZHU, W. ET AL.: "Kinetic properties of polymorphic variants and pathogenic mutants in human cystathionine gamma-lyase", BIOCHEMISTRY, vol. 47, no. 23, 14 May 2008 (2008-05-14), pages 6226 - 6232, XP055515416
KRAUS, J. P ET AL.: "Cystathionine gamma-lyase: Clinical, metabolic, genetic, and structural studies", MOLECULAR GENETICS AND METABOLISM, vol. 97, no. 4, 9 April 2009 (2009-04-09), pages 250 - 259, XP026282971
LU , Y. ET AL.: "Cloning and nucleotide sequence of human liver cDNA encoding for cystathionine gamma-lyase", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 189, no. 2, 15 December 1992 (1992-12-15), pages 749 - 758, XP024838385
BREITINGER, U. ET AL.: "The three-dimensional structure of cystathionine beta -lyase from Arabidopsis and its substrate specificity", PLANT PHYSIOLOGY, vol. 126, no. 2, June 2001 (2001-06-01), pages 631 - 642, XP055515421
KRAUS, J. P ET AL.: "Cystathionine gamma-lyase: Clinical, metabolic, genetic, and structural studies", MOLECULAR GENETICS AND METABOLISM, vol. 97, no. 4, 9 April 2009 (2009-04-09), pages 250 - 259, XP026282971
LU , Y. ET AL.: "Cloning and nucleotide sequence of human liver cDNA encoding for cystathionine gamma-lyase", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 189, no. 2, 15 December 1992 (1992-12-15), pages 749 - 758, XP024838385
BREITINGER, U. ET AL.: "The three-dimensional structure of cystathionine beta -lyase from Arabidopsis and its substrate specificity", PLANT PHYSIOLOGY, vol. 126, no. 2, June 2001 (2001-06-01), pages 631 - 642, XP055515421
Attorney, Agent or Firm:
BARRETT, Tamsen, L. (US)
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