Title:
ENGINEERED SECRETED ALKALINE PHOSPHATASE (SEAP) REPORTER GENES AND POLYPEPTIDES
Document Type and Number:
WIPO Patent Application WO2002095068
Kind Code:
A3
Abstract:
The invention provides a novel reporter gene (mSEAP) capable of being expressed in a mammal for extended periods of time. Nucleic acids encoding the reporter gene, cells and vectors comprising the nucleic acids, and methods of using the reporter gene to identify expression vectors and screen for drug compounds in an animal are also disclosed.
Inventors:
THUILLIER (FR)
WANG MANPING (US)
ORSINI CECILE (FR)
WANG MANPING (US)
ORSINI CECILE (FR)
Application Number:
PCT/EP2002/006930
Publication Date:
March 11, 2004
Filing Date:
May 27, 2002
Export Citation:
Assignee:
GENCELL SA (FR)
THUILLIER (FR)
WANG MANPING (US)
ORSINI CECILE (FR)
THUILLIER (FR)
WANG MANPING (US)
ORSINI CECILE (FR)
International Classes:
C12N5/10; C12N9/16; A01K67/027; C12N15/09; C12Q1/68; C12Q1/6897; (IPC1-7): C12Q1/68; C12N9/16
Foreign References:
| EP0327960A1 | 1989-08-16 |
Other References:
BETTAN M. ET AL.,: "secreted human placental alkaline phosphatase as a reporter gne or in vivo gene transfer", ANAL. BIOCHEMISTRY, vol. 271, - 1999, pages 187 - 189, XP002191938
GREGORY B ET AL: "A FAST AND SENSITIVE COLORIMETRIC ASSAY FOR IL-6 IN HEPATOMA CELLS BASED ON THE PRODUCTION OF A SECRETED FORM OF ALKALINE PHOSPHATASE (SEAP)9", JOURNAL OF IMMUNOLOGICAL METHODS, ELSEVIER SCIENCE PUBLISHERS B.V.,AMSTERDAM, NL, vol. 170, 29 March 1994 (1994-03-29), pages 47 - 56, XP000601567, ISSN: 0022-1759
BARTLETT R J ET AL: "LONG-TERM EXPRESSION OF A FLUORESCENT REPORTER GENE VIA DIRECT INJECTION OF PLASMID VECTOR INTO MOUSE SKELETAL MUSCLE: COMPARISON OF HUMAN CREATINE KINASE AND CMV PROMOTER EXPRESSION LEVELS IN VIVO", CELL TRANSPLANTATION, ELSEVIER SCIENCE, US, vol. 5, no. 3, 1996, pages 411 - 419, XP002920729, ISSN: 0963-6897
MANES T ET AL: "GENOMIC STRUCTURE AND COMPARISON OF MOUSE TISSUE-SPECIFIC ALKALINE PHOSPHATASE GENES", GENOMICS, ACADEMIC PRESS, SAN DIEGO, US, vol. 8, 1990, pages 541 - 554, XP001062172, ISSN: 0888-7543
WANG M. ET AL.,: "MUSEAP, a novel reporter gene for the study of long-term gene expression in immunocompetent mice", GENE, vol. 279, 14 November 2001 (2001-11-14), pages 99 - 108, XP004324228
GREGORY B ET AL: "A FAST AND SENSITIVE COLORIMETRIC ASSAY FOR IL-6 IN HEPATOMA CELLS BASED ON THE PRODUCTION OF A SECRETED FORM OF ALKALINE PHOSPHATASE (SEAP)9", JOURNAL OF IMMUNOLOGICAL METHODS, ELSEVIER SCIENCE PUBLISHERS B.V.,AMSTERDAM, NL, vol. 170, 29 March 1994 (1994-03-29), pages 47 - 56, XP000601567, ISSN: 0022-1759
BARTLETT R J ET AL: "LONG-TERM EXPRESSION OF A FLUORESCENT REPORTER GENE VIA DIRECT INJECTION OF PLASMID VECTOR INTO MOUSE SKELETAL MUSCLE: COMPARISON OF HUMAN CREATINE KINASE AND CMV PROMOTER EXPRESSION LEVELS IN VIVO", CELL TRANSPLANTATION, ELSEVIER SCIENCE, US, vol. 5, no. 3, 1996, pages 411 - 419, XP002920729, ISSN: 0963-6897
MANES T ET AL: "GENOMIC STRUCTURE AND COMPARISON OF MOUSE TISSUE-SPECIFIC ALKALINE PHOSPHATASE GENES", GENOMICS, ACADEMIC PRESS, SAN DIEGO, US, vol. 8, 1990, pages 541 - 554, XP001062172, ISSN: 0888-7543
WANG M. ET AL.,: "MUSEAP, a novel reporter gene for the study of long-term gene expression in immunocompetent mice", GENE, vol. 279, 14 November 2001 (2001-11-14), pages 99 - 108, XP004324228
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