Title:
ENGINEERED SUBTILIGASE VARIANTS FOR VERSATILE, SITE-SPECIFIC LABELING OF PROTEINS
Document Type and Number:
WIPO Patent Application WO/2018/045200
Kind Code:
A3
Abstract:
In various embodiments, the invention provides subtiligase variants that recognize an N- terminus of a protein or peptide substrate that is different than the N-terminus that is recognized by the corresponding wild-type subtiligase. Also provided are methods and kits for using such subtiligase variants to site-specifically ligate a synthetic molecule comprising a peptide ester to a protein or peptide substrate of interest.
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Inventors:
WELLS JAMES (US)
WEEKS AMY (US)
WEEKS AMY (US)
Application Number:
PCT/US2017/049670
Publication Date:
April 19, 2018
Filing Date:
August 31, 2017
Export Citation:
Assignee:
THE REGENT OF THE UNIV OFCALIFORNIA (US)
International Classes:
C07K1/02; C07K1/36; C07K14/32
Domestic Patent References:
| WO2016056913A1 | 2016-04-14 |
Foreign References:
| US20030077807A1 | 2003-04-24 |
Other References:
WEEKS ET AL.: "Engineering peptide ligase specificity by proteomic identification of ligation sites", NATURE CHEMICAL BIOLOGY, vol. 14, no. 1, 20 November 2017 (2017-11-20), pages 50 - 57, XP055475264
Attorney, Agent or Firm:
MANN, Jeffry, S. et al. (US)
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