Title:
ENGINEERING AN INCREASE IN ETHANOL PRODUCTION BY ALTERING COFACTOR SPECIFICITY
Document Type and Number:
WIPO Patent Application WO/2013/141905
Kind Code:
A3
Abstract:
The present invention provides for the manipulation of cofactor usage in a recombinant host cell to increase the formation of desirable products. In some embodiments, the invention provides for a recombinant microorganism comprising a mutation in one or more native enzymes such that their cofactor specificity is altered in such a way that overall cofactor usage in the cell is balanced for a specified pathway and there is an increase in a specific product formation within the cell. In some embodiments, endogenous enzymes are replaced by enzymes with an alternate cofactor specificity from a different species.
Inventors:
GUSS ADAM M (US)
LO JONATHAN (US)
VAN DIJKEN JOHANNES P (NL)
SHAW IV ARTHUR J (US)
OLSON DANIEL G (US)
HERRING CHRISTOPHER D (US)
LO JONATHAN (US)
VAN DIJKEN JOHANNES P (NL)
SHAW IV ARTHUR J (US)
OLSON DANIEL G (US)
HERRING CHRISTOPHER D (US)
Application Number:
PCT/US2012/067216
Publication Date:
March 13, 2014
Filing Date:
November 30, 2012
Export Citation:
Assignee:
MASCOMA CORP (US)
DARTMOUTH COLLEGE (US)
GUSS ADAM M (US)
DARTMOUTH COLLEGE (US)
GUSS ADAM M (US)
International Classes:
C12N9/04; C12N9/02; C12P7/06
Domestic Patent References:
| WO2010051527A2 | 2010-05-06 | |||
| WO2010010116A1 | 2010-01-28 |
Foreign References:
| EP2277989A1 | 2011-01-26 |
Other References:
SABINE BASTIAN ET AL: "Engineered ketol-acid reductoisomerase and alcohol dehydrogenase enable anaerobic 2-methylpropan-1-ol production at theoretical yield in", METABOLIC ENGINEERING, vol. 13, no. 3, 16 February 2011 (2011-02-16), pages 345 - 352, XP028197952, ISSN: 1096-7176, [retrieved on 20110216], DOI: 10.1016/J.YMBEN.2011.02.004
CAMPBELL ELLIOT ET AL: "Broadening the cofactor specificity of a thermostable alcohol dehydrogenase using rational protein design introduces novel kinetic transient behavior.", BIOTECHNOLOGY AND BIOENGINEERING 1 DEC 2010, vol. 107, no. 5, 1 December 2010 (2010-12-01), pages 763 - 774, XP002711509, ISSN: 1097-0290
MATSUSHIKA A ET AL: "Bioethanol production from xylose by recombinant Saccharomyces cerevisiae expressing xylose reductase, NADP<+>-dependent xylitol dehydrogenase, and xylulokinase", JOURNAL OF BIOSCIENCE AND BIOENGINEERING, ELSEVIER, AMSTERDAM, NL, vol. 105, no. 3, 1 March 2008 (2008-03-01), pages 296 - 299, XP022590047, ISSN: 1389-1723, [retrieved on 20080301], DOI: 10.1263/JBB.105.296
BROWN STEVEN D ET AL: "Mutant alcohol dehydrogenase leads to improved ethanol tolerance in Clostridium thermocellum.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 16 AUG 2011, vol. 108, no. 33, 16 August 2011 (2011-08-16), pages 13752 - 13757, XP002711510, ISSN: 1091-6490
CAMPBELL ELLIOT ET AL: "Broadening the cofactor specificity of a thermostable alcohol dehydrogenase using rational protein design introduces novel kinetic transient behavior.", BIOTECHNOLOGY AND BIOENGINEERING 1 DEC 2010, vol. 107, no. 5, 1 December 2010 (2010-12-01), pages 763 - 774, XP002711509, ISSN: 1097-0290
MATSUSHIKA A ET AL: "Bioethanol production from xylose by recombinant Saccharomyces cerevisiae expressing xylose reductase, NADP<+>-dependent xylitol dehydrogenase, and xylulokinase", JOURNAL OF BIOSCIENCE AND BIOENGINEERING, ELSEVIER, AMSTERDAM, NL, vol. 105, no. 3, 1 March 2008 (2008-03-01), pages 296 - 299, XP022590047, ISSN: 1389-1723, [retrieved on 20080301], DOI: 10.1263/JBB.105.296
BROWN STEVEN D ET AL: "Mutant alcohol dehydrogenase leads to improved ethanol tolerance in Clostridium thermocellum.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 16 AUG 2011, vol. 108, no. 33, 16 August 2011 (2011-08-16), pages 13752 - 13757, XP002711510, ISSN: 1091-6490
Attorney, Agent or Firm:
JACKMAN, Peter, A. et al. (Kessler Goldstein & Fox PLLC,1100 New York Avenue N.W, Washington District of Columbia, US)
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